Hosseini Seyed Ruhollah, Kaka Gholamreza, Joghataei Mohammad Taghi, Hooshmandi Mehdi, Sadraie Seyed Homayoon, Yaghoobi Kayvan, Mansoori Korosh, Mohammadi Alireza
Neuroscience Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Cell J. 2017 Apr-Jun;19(1):102-116. doi: 10.22074/cellj.2016.4868. Epub 2016 Dec 21.
Spinal cord injury (SCI) causes inflammation, deformity and cell loss. It has been shown that (MO), as herbal medicine, and dexamethasone (DEX) are useful in the prevention of various neurological diseases. The present study evaluated combinational effects of DEX and MO on spinal cord injury.
Thirty six adult male Wistar rats were used in this experimental study. The weight-drop contusion method was employed to induce spinal cord injury in rats. DEX and MO were administrated alone and together in different treatment groups. Intra-muscular injection of DEX (1 mg/kg) was started three hours after injury and continued once a day for seven days after injury. Intra-peritoneal (I.P) injection of MO (150 mg/ kg) was started one day after injury and continued once a day for 14 days.
Our results showed motor and sensory functions were improved significantly in the group received a combination of DEX and MO, compared to spinal cord injury group. Mean cavity area was decreased and loss of lower motor neurons and astrogliosis in the ventral horn of spinal cord was significantly prevented in the group received combination of DEX and , compared to spinal cord injury group. Furthermore, the findings showed a significant augmentation of electromyography (EMG) recruitment index, increase of myelin diameter, and up-regulation of myelin basic protein in the treated group with combination of DEX and MO.
Results showed that combination of DEX and MO could be considered as a neuroprotective agent in spinal cord injury.
脊髓损伤（SCI）会引发炎症、畸形和细胞损失。已有研究表明，月见草油（MO）作为一种草药，以及地塞米松（DEX）在预防各种神经疾病方面具有一定作用。本研究评估了DEX和MO联合使用对脊髓损伤的影响。
本实验研究使用了36只成年雄性Wistar大鼠。采用重物坠落挫伤法诱导大鼠脊髓损伤。DEX和MO在不同治疗组中单独及联合使用。损伤后3小时开始肌肉注射DEX（1mg/kg），并在损伤后持续每日注射一次，共7天。损伤后1天开始腹腔注射MO（150mg/kg），并持续每日注射一次，共14天。
我们的结果显示，与脊髓损伤组相比，接受DEX和MO联合治疗的组运动和感觉功能有显著改善。与脊髓损伤组相比，接受DEX和MO联合治疗的组平均空洞面积减小，脊髓腹角下运动神经元损失和星形胶质细胞增生得到显著预防。此外，研究结果显示，在接受DEX和MO联合治疗的组中，肌电图（EMG）募集指数显著增加，髓鞘直径增大，髓鞘碱性蛋白上调。
结果表明，DEX和MO联合使用可被视为脊髓损伤的一种神经保护剂。
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