Rastegarian Ahmad, Abedi Hassanali, Jahromi Hossein K, Zarei Safar, Nematollahi Arash, Mansouri Esrafil, Sameni Hadi
Research Center for Noncommunicable Diseases, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
Research Center for Noncommunicable Diseases, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
J Acupunct Meridian Stud. 2020 Feb;13(1):19-24. doi: 10.1016/j.jams.2019.11.001. Epub 2019 Nov 16.
Melissa officinalis (MO) is one of the oldest herbal medicines commonly used in traditional medicine, which some studies have investigated for its analgesic effect. This study is an attempt to investigate the effects of intrathecal administration of Melissa officinalis on the pain induced by heat and formalin. In this experimental study, 70 male Wistar rats with an average weight of 270-320 g were randomly divided into five groups: control; sham that received 25 μl of saline through the spinal catheter; and three experimental groups that received 5, 10 or 20 mg/kg M. officinalis via the spinal catheter respectively. Five days after catheterization of the spinal cord from the lumbar region under anesthesia, the effects of Intrathecal administration of M. officinalis on heat- and formalin-induced pain were evaluated. Data were analyzed by using one-way ANOVA. Intrathecal injection of M. officinalis blocked heat-induced pain compared to sham group (p = 0.001). Maximum analgesia was observed 30 min after the injection. Furthermore, intrathecal administration of MO alleviated both acute (p = 0.007) and chronic (p = 0.001) phases of formalin-induced pain. Motor block was not observed in any of the above mentioned groups. The results showed that intrathecal administration of MO could significantly improve hot-water and formalin-induced pain in male Wistar rats.
蜜蜂花（MO）是传统医学中常用的最古老的草药之一，一些研究对其镇痛作用进行了调查。本研究旨在探讨鞘内注射蜜蜂花对热和福尔马林诱导的疼痛的影响。在这项实验研究中，70只平均体重为270 - 320克的雄性Wistar大鼠被随机分为五组：对照组；假手术组，通过脊髓导管接受25微升生理盐水；三个实验组，分别通过脊髓导管接受5、10或20毫克/千克的蜜蜂花。在麻醉下从腰椎区域进行脊髓导管插入术后五天，评估鞘内注射蜜蜂花对热和福尔马林诱导的疼痛的影响。数据采用单因素方差分析进行分析。与假手术组相比，鞘内注射蜜蜂花可阻断热诱导的疼痛（p = 0.001）。注射后30分钟观察到最大镇痛效果。此外，鞘内注射蜜蜂花可减轻福尔马林诱导疼痛的急性（p = 0.007）和慢性（p = 0.001）阶段。在上述任何一组中均未观察到运动阻滞。结果表明，鞘内注射蜜蜂花可显著改善雄性Wistar大鼠的热水和福尔马林诱导的疼痛。
