Yoshida Atsumi, Watanabe Kazushi, Iwasaki Ai, Kimura Chiharu, Matsushita Hiroshi, Wakatsuki Akihiko
a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
J Matern Fetal Neonatal Med. 2018 Apr;31(8):1051-1057. doi: 10.1080/14767058.2017.1306510. Epub 2017 Apr 3.
The purpose of this study was to investigate the relationship between placental oxidative stress and maternal endothelial function in pregnant women with normotensive fetal growth restriction (FGR).
We examined serum concentrations of oxygen free radicals (d-ROMs), maternal angiogenic factor (PlGF), and sFlt-1, placental oxidative DNA damage, and maternal endothelial function in 17 women with early-onset preeclampsia (PE), 18 with late-onset PE, 14 with normotensive FGR, and 21 controls. Flow-mediated vasodilation (FMD) was assessed as a marker of maternal endothelial function. Immunohistochemical analysis was performed to measure the proportion of placental trophoblast cell nuclei staining positive for 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage.
Maternal serum d-ROM, sFlt-1 concentrations, and FMD did not significantly differ between the control and normotensive FGR groups. The proportion of nuclei staining positive for 8-OHdG was significantly higher in the normotensive FGR group relative to the control group.
Our findings demonstrate that, despite the presence of placental oxidative DNA damage as observed in PE patients, pregnant women with normotensive FGR show no increase in the concentrations of sFlt-1 and d-ROMs, or a decrease in FMD.
本研究旨在探讨血压正常的胎儿生长受限（FGR）孕妇胎盘氧化应激与母体内皮功能之间的关系。
我们检测了17例早发型子痫前期（PE）孕妇、18例晚发型PE孕妇、14例血压正常的FGR孕妇和21例对照组孕妇的血清氧自由基（d-ROMs）浓度、母体血管生成因子（PlGF）和可溶性血管内皮生长因子受体-1（sFlt-1）、胎盘氧化DNA损伤以及母体内皮功能。采用血流介导的血管舒张功能（FMD）作为母体内皮功能的标志物进行评估。进行免疫组织化学分析以测量胎盘滋养层细胞核中8-羟基-2'-脱氧鸟苷（8-OHdG，氧化DNA损伤的标志物）染色阳性的比例。
对照组和血压正常的FGR组之间，母体血清d-ROM、sFlt-1浓度和FMD无显著差异。与对照组相比，血压正常的FGR组中8-OHdG染色阳性的细胞核比例显著更高。
我们的研究结果表明，尽管在PE患者中观察到胎盘存在氧化DNA损伤，但血压正常的FGR孕妇的sFlt-1和d-ROMs浓度并未升高，FMD也未降低。
