Cha Jeong-Dan, Jung Eun-Kyung, Choi Sung-Mi, Lee Kyung-Yeol, Kang Sung-Wook
Department of Oral Microbiology and Institute of Oral Bioscience, Chonbuk National University.
J Oral Sci. 2017;59(1):31-38. doi: 10.2334/josnusd.16-0150.
Drynaria fortunei (D. fortunei), widely used in traditional Korean medicine, is reportedly effective in treating inflammation, hyperlipidemia, bone fractures, oxidative damage, arteriosclerosis, rheumatism, and gynecological diseases. The objective of this study was to evaluate the antibacterial effects of the chloroform fraction of D. fortunei (DFCF) and assess the synergistic effects of DFCF with antibiotics against bacterial pathogens. This was carried out by calculating the minimal inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) and performing checkerboard dilution test and time-kill assays. The MICs/MBCs for DFCF, ampicillin, and gentamicin against all oral strains were >39-2,500/5,000 μg/mL, 0.25-64/0.25-64 µg/mL, and 0.5-256/1-512 µg/mL, respectively. DFCF exhibited the highest activity against the periodontic pathogens Prevotella intermedia and Porphylomonas gingivalis. DFCF in combination with ampicillin showed a strong synergistic effect against oral bacteria (fractional inhibitory concentration (FIC) index ≤0.5), whereas on combining with gentamicin, it reduced the on half-eighth times than used alone (FICI ≤ 0.5). DFCF combined with ampicillin or gentamicin killed 100% of most tested bacteria within 3-4 h. The results of this study demonstrate the antimicrobial and synergistic activity of DFCF and antibiotics against oral pathogens.
骨碎补在传统韩医学中应用广泛，据报道其在治疗炎症、高脂血症、骨折、氧化损伤、动脉硬化、风湿和妇科疾病方面具有疗效。本研究的目的是评估骨碎补氯仿提取物（DFCF）的抗菌作用，并评估DFCF与抗生素联合对细菌病原体的协同作用。这通过计算最低抑菌浓度（MICs）和最低杀菌浓度（MBCs）以及进行棋盘稀释试验和时间杀菌试验来实现。DFCF、氨苄西林和庆大霉素对所有口腔菌株的MICs/MBCs分别>39 - 2,500/5,000 μg/mL、0.25 - 64/0.25 - 64 μg/mL和0.5 - 256/1 - 512 μg/mL。DFCF对牙周病原体中间普氏菌和牙龈卟啉单胞菌表现出最高活性。DFCF与氨苄西林联合对口腔细菌显示出强烈的协同作用（部分抑菌浓度（FIC）指数≤0.5），而与庆大霉素联合时，其杀菌效果比单独使用时提高了八倍（FICI≤0.5）。DFCF与氨苄西林或庆大霉素联合在3 - 4小时内杀死了大多数受试细菌的100%。本研究结果证明了DFCF和抗生素对口腔病原体的抗菌和协同活性。
