Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
Pathog Dis. 2017 Jun 1;75(4). doi: 10.1093/femspd/ftx037.
Achromobacter insolitus is a newly described, Gram-negative, small (1-2 mm long) and coccoid pathogen that has been isolated from leg wound. Due to its potential threat to human beings, the type strain of this species LMG 6003T was completely sequenced in this study. The genomic analysis revealed that the genome of LMG 6003T consists of one circular DNA chromosome of 6 492 697 bp with a GC content of 65.10%. The entire genome contains 6208 predicted coding genes, 61 tRNAs and 13 rRNA genes. Comparative genome analysis between LMG 6003T and another selected 10 sequenced Achromobacter revealed that 725 genes only exist in LMG 6003T. In particular, these genes include several important pathogenic-related genes such as Type IV prepilin peptidase (TadV/CpaA), Type VI secretion lipoprotein (VasD) and type 1 fimbriae major subunit (FimA). Genomic island (GI) analysis also suggests the existence of GIs in LMG 6003T. All these results strongly suggest the unique genomic features in LMG 6003T against other Achromobacter species. This report will provide us with an extended understanding of A. insolitus at the genomic level and would be helpful for understanding the evolution of the Achromobacter genus.
不可培养阿克曼氏菌是一种新描述的革兰氏阴性、小（1-2 毫米长）和球形的病原体，已从腿部伤口中分离出来。由于其对人类的潜在威胁，本研究对该物种的模式株 LMG 6003T 进行了全基因组测序。基因组分析表明，LMG 6003T 的基因组由一个 6492697 bp 的圆形 DNA 染色体组成，GC 含量为 65.10%。整个基因组包含 6208 个预测编码基因、61 个 tRNA 和 13 个 rRNA 基因。与其他 10 株已测序的不可培养阿克曼氏菌的比较基因组分析表明，725 个基因仅存在于 LMG 6003T 中。特别是，这些基因包括一些重要的与致病性相关的基因，如 IV 型前导肽酶（TadV/CpaA）、VI 型分泌脂蛋白（VasD）和 1 型菌毛主要亚基（FimA）。基因组岛（GI）分析还表明 LMG 6003T 中存在 GI。所有这些结果强烈表明 LMG 6003T 相对于其他不可培养阿克曼氏菌具有独特的基因组特征。本报告将在基因组水平上为我们提供对不可培养阿克曼氏菌的更深入了解，并有助于理解阿克曼氏菌属的进化。
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