Al-Kahachi Rusul, Al-Asadi Sura, Ali Zainab Oun, Rampurawala Jamila
Department of Scholarships and Cultural Relationship, Ministry of Higher Education and Scientific Research, Baghdad, Iraq.
Department of Laboratory Techniques, College of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq.
Iran J Microbiol. 2023 Jun;15(3):414-424. doi: 10.18502/ijm.v15i3.12902.
The study aimed to investigate whether IA strain biofilm formation, which contributes to antibiotic resistance, could be enhanced by readily available nutrient sources like carbohydrates and environmental factors such as pH and NaCl. Additionally, the study aimed to identify any inherent genes that support biofilm formation in this strain, which is an opportunistic pathogen that affects immunocompromised patients and is resistant to many antibiotics.
Biofilm growth in different carbohydrate, pH, and NaCl concentrated media was measured using crystal violet microtiter assay. All the treatments were subjected to biostatistics analysis for normality, Test of Homogeneity, one way ANOVA analysis. Whole-genome sequencing of our IA strain was conducted to identify various gene sequences.
Biofilm formation was measured at different carbohydrate concentrations, and the optimum biofilm formation was observed at 3M glucose and 0.5M NaCl, while the lowest results were seen at 2M maltose concentration. Whole-genome sequencing identified potential genes involved in biofilm formation, pathogenicity, protein metabolism, flagellar motility, cell wall component synthesis, and a multidrug efflux pump.
These findings suggest that biofilm formation is influenced by extrinsic and intrinsic factors, which could aid in the development of effective treatments for resistant infections.
本研究旨在调查有助于抗生素耐药性的IA菌株生物膜形成是否会因碳水化合物等易于获取的营养源以及pH值和氯化钠等环境因素而增强。此外，本研究旨在确定该菌株中支持生物膜形成的任何固有基因，该菌株是一种影响免疫功能低下患者且对多种抗生素耐药的机会致病菌。
使用结晶紫微量滴定法测量不同碳水化合物、pH值和氯化钠浓缩培养基中的生物膜生长情况。所有处理均进行正态性、齐性检验、单向方差分析的生物统计学分析。对我们的IA菌株进行全基因组测序以鉴定各种基因序列。
在不同碳水化合物浓度下测量生物膜形成情况，在3M葡萄糖和0.5M氯化钠时观察到最佳生物膜形成，而在2M麦芽糖浓度时结果最低。全基因组测序确定了参与生物膜形成、致病性、蛋白质代谢、鞭毛运动、细胞壁成分合成和多药外排泵的潜在基因。
这些发现表明生物膜形成受外在和内在因素影响，这可能有助于开发针对耐药感染的有效治疗方法。
