Hedenborg G, Norman A, Ritzén A
Department of Clinical Chemistry, Karolinska sjukhuset, Stockholm, Sweden.
Scand J Clin Lab Invest. 1988 May;48(3):241-5. doi: 10.3109/00365518809167490.
Individual bile acids were determined by gas-liquid chromatography in, very low density, low density and high density lipoprotein fractions obtained by sequential ultracentrifugation of serum from normal adults, both postprandially and during fasting. The lipoproteins were found to contain 22-34% of fasting serum bile acids. The observed postprandial increase in bile acids did not exhibit any shift in the ratio between lipoprotein bound- and non-lipoprotein-bound bile acids. Bile acids were present in all isolated lipoprotein fractions, high density lipoproteins containing the highest amounts. In the lipoprotein fraction, a higher percentage of cholate than of chenodeoxycholate was found.
通过对正常成年人血清进行连续超速离心，分别在餐后和空腹状态下获得极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白组分，采用气液色谱法测定其中的单个胆汁酸。发现这些脂蛋白含有空腹血清胆汁酸的22%-34%。观察到的餐后胆汁酸增加并未表现出脂蛋白结合型胆汁酸与非脂蛋白结合型胆汁酸之间比例的任何变化。胆汁酸存在于所有分离出的脂蛋白组分中，其中高密度脂蛋白所含胆汁酸量最高。在脂蛋白组分中，发现胆酸盐的百分比高于鹅去氧胆酸盐。
