Leelatian Nalin, Doxie Deon B, Greenplate Allison R, Sinnaeve Justine, Ihrie Rebecca A, Irish Jonathan M
Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee.
Department of Pathology, Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee.
Curr Protoc Mol Biol. 2017 Apr 3;118:25C.1.1-25C.1.23. doi: 10.1002/cpmb.37.
Mass cytometry is a single-cell biology technique that samples >500 cells per second, measures >35 features per cell, and is sensitive across a dynamic range of >10 relative intensity units per feature. This combination of technical assets has powered a series of recent cytomic studies where investigators used mass cytometry to measure protein and phospho-protein expression in millions of cells, characterize rare cell types in healthy and diseased tissues, and reveal novel, unexpected cells. However, these advances largely occurred in studies of blood, lymphoid tissues, and bone marrow, since the cells in these tissues are readily obtained in single-cell suspensions. This unit establishes a primer for single-cell analysis of solid tumors and tissues, and has been tested with mass cytometry. The cells obtained from these protocols can be fixed for study, cryopreserved for long-term storage, or perturbed ex vivo to dissect responses to stimuli and inhibitors. © 2017 by John Wiley & Sons, Inc.
质谱流式细胞术是一种单细胞生物学技术，每秒可对超过500个细胞进行采样，每个细胞可测量超过35个特征，并且对每个特征超过10个相对强度单位的动态范围敏感。这些技术优势的结合推动了一系列近期的细胞组学研究，研究人员使用质谱流式细胞术来测量数百万个细胞中的蛋白质和磷酸化蛋白质表达，表征健康和患病组织中的稀有细胞类型，并揭示新的、意想不到的细胞。然而，这些进展主要发生在血液、淋巴组织和骨髓的研究中，因为这些组织中的细胞很容易以单细胞悬液的形式获得。本单元为实体瘤和组织的单细胞分析建立了一个入门指南，并已通过质谱流式细胞术进行了测试。从这些方案中获得的细胞可以固定用于研究，冷冻保存用于长期储存，或在体外进行扰动以剖析对刺激物和抑制剂的反应。© 2017约翰威立国际出版公司
