神经节苷脂GM1治疗可预防亚急性接触甲苯对大鼠纹状体膜中N-[3H]丙基去甲阿朴吗啡结合特性的影响。

Ganglioside GM1 treatment prevents the effects of subacute exposure to toluene on N-[3H]propylnorapomorphine binding characteristics in rat striatal membranes.

作者信息

von Euler G, Fuxe K, Agnati L F, Hansson T, Gustafsson J A

机构信息

Department of Histology, Karolinska Institute, Stockholm, Sweden.

出版信息

Neurosci Lett. 1987 Nov 23;82(2):181-4. doi: 10.1016/0304-3940(87)90126-1.

DOI:10.1016/0304-3940(87)90126-1

PMID:3696491

Abstract

The effects of ganglioside GM1 treatment (10 mg/kg, i.p., 3 days, once daily) were analyzed on N-[3H]propylnorapomorphine ([3H]NPA) binding characteristics in striatal membrane preparations from air or toluene (80 ppm, 3 days, 6 h/day) exposed adult male rats. It was found that toluene exposure induced increased Kd and Bmax values for [3H]NPA binding. These changes could be blocked by GM1 treatment, which by itself had no significant effects on the binding characteristics of [3H]NPA. The addition of toluene (9.3 mumol/ml) in vitro had similar effects on [3H]NPA binding characteristics as toluene exposure in vivo. These results indicate that the effects of toluene exposure on dopamine receptors are due to an alteration of membrane fluidity which is prevented by GM1 treatment.

摘要

分析了神经节苷脂GM1治疗（10毫克/千克，腹腔注射，3天，每日一次）对成年雄性大鼠暴露于空气或甲苯（80 ppm，3天，每天6小时）后纹状体膜制剂中N-[³H]丙基去甲阿扑吗啡（[³H]NPA）结合特性的影响。发现甲苯暴露导致[³H]NPA结合的解离常数（Kd）和最大结合容量（Bmax）值增加。这些变化可被GM1治疗阻断，而GM1本身对[³H]NPA的结合特性无显著影响。体外添加甲苯（9.3微摩尔/毫升）对[³H]NPA结合特性的影响与体内甲苯暴露相似。这些结果表明，甲苯暴露对多巴胺受体的影响是由于膜流动性的改变，而GM1治疗可防止这种改变。