Fiedurek Jan, Trytek Mariusz, Szczodrak Janusz
Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
J Basic Microbiol. 2017 Jun;57(6):445-459. doi: 10.1002/jobm.201600710. Epub 2017 Mar 28.
Improvement of the biosynthetic capabilities of industrially relevant microbes to produce desired metabolites in higher quantities is one of the important topics of modern biotechnology. In this article, different strategies of improvement of mutated microbial strains are briefly described. This is followed by the first comprehensive review of the literature on obtaining high yielding microorganisms, that is, mutants exhibiting resistance to antimetabolites, nutritional repression, and abiotic stresses as well as tolerance to solvents and toxic substrates or products. Furthermore, the efficiency of the microbial metabolites produced by improved microbial strains, advantages, and limitations, as well as future prospects for strategies of strain development are discussed.
提高具有工业相关性的微生物的生物合成能力，以大量生产所需代谢物，是现代生物技术的重要课题之一。本文简要描述了改良突变微生物菌株的不同策略。随后是对关于获得高产微生物文献的首次全面综述，即对抗代谢物、营养抑制和非生物胁迫具有抗性，以及对溶剂、有毒底物或产物具有耐受性的突变体。此外，还讨论了改良微生物菌株产生的微生物代谢物的效率、优点和局限性，以及菌株开发策略的未来前景。
