Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
Angew Chem Int Ed Engl. 2017 Apr 18;56(17):4802-4805. doi: 10.1002/anie.201702415. Epub 2017 Mar 28.
The monitoring of cancer biomarkers is crucial to the early detection of cancer. However, a limiting factor in biomarker analysis is the ability to obtain the multilayered information of various biomarker molecules located at different parts of cells from the plasma membrane to the cytoplasm. A two-stage dissociation nanoparticle system based on multifunctionalized polydopamine-coated gold nanoparticles (Au@PDA NPs) is reported, which allows for the two-stage imaging of cancer biomarkers in single cells. We demonstrate the feasibility of this strategy on sialic acids (SAs), p53 protein, and microRNA-21 (miRNA-21) in MCF-7 breast cancer cells by two custom-built probes. Furthermore, the multicolor fluorescence information extracted is used for the monitoring of biomarker expression changes under different drug combinations, which allows us to investigate the complex interactions between various cancer biomarkers and to describe the cancer biomarker-synergic networks in single cells.
癌症生物标志物的监测对于癌症的早期发现至关重要。然而，生物标志物分析的一个限制因素是从质膜到细胞质的不同位置的各种生物标志物分子的多层信息的获取能力。本文报道了一种基于多功能化聚多巴胺包覆的金纳米粒子(Au@PDA NPs)的两阶段解离纳米粒子系统，该系统允许在单细胞中对癌症生物标志物进行两阶段成像。我们通过两个定制的探针，在 MCF-7 乳腺癌细胞中的唾液酸（SAs）、p53 蛋白和 microRNA-21（miRNA-21）上证明了该策略的可行性。此外，提取的多色荧光信息用于监测不同药物组合下生物标志物表达的变化，这使我们能够研究各种癌症生物标志物之间的复杂相互作用，并在单细胞中描述癌症生物标志物协同网络。
