蛋白质与改性细菌纤维素纳米纤维的亲和结合。
Affinity binding of proteins to the modified bacterial cellulose nanofibers.
作者信息
Bakhshpour Monireh, Tamahkar Emel, Andaç Müge, Denizli Adil
机构信息
Hacettepe University, Chemistry Department, 06800 Ankara, Turkey.
Hacettepe University, Chemistry Department, 06800 Ankara, Turkey; Hitit University, Chemical Engineering Department, 19030 Çorum, Turkey.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 May 1;1052:121-127. doi: 10.1016/j.jchromb.2017.03.021. Epub 2017 Mar 27.
The potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-l-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed.
通过金属离子配位相互作用来增强蛋白质吸附和结合能力，测定了改性细菌纤维素（BC）纳米纤维的潜力。因此，分别使用一种家用合成的金属螯合单体，即N-甲基丙烯酰基-L-组氨酸甲酯（MAH），和一种商业金属螯合单体，即4-乙烯基咪唑（VIm），与金属离子Cu（II）和Ni（II）络合，用于合成改性BC纳米纤维。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和能谱分析（EDX）测量对改性纳米纤维进行了表征。以血红蛋白作为模型蛋白进行了蛋白质吸附试验，结果表明血红蛋白在改性BC纳米纤维上的最大吸附容量为47.40mg/g。开发了制备金属螯合纳米纤维的新策略。
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