采用 QuEChERS 法和液相色谱-串联质谱法测定植物性饮料中的真菌毒素。
Determination of mycotoxins in plant-based beverages using QuEChERS and liquid chromatography-tandem mass spectrometry.
机构信息
Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo, s/n, 43007 Tarragona, Spain; Group of Research on Omic Methodologies (GROM), Centre for Omic Sciences (COS), Universitat Rovira i Virgili, Av. Universitat 1, 43204 Reus, Spain.
Group of Research on Omic Methodologies (GROM), Centre for Omic Sciences (COS), Universitat Rovira i Virgili, Av. Universitat 1, 43204 Reus, Spain.
出版信息
Food Chem. 2017 Aug 15;229:366-372. doi: 10.1016/j.foodchem.2017.02.078. Epub 2017 Feb 17.
A method was developed for the simultaneous determination of 11 mycotoxins in plant-based beverage matrices, using a QuEChERS extraction followed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry detection (UHPLC-(ESI)MS/MS). This multi-mycotoxin method was applied to analyse plant-based beverages such as soy, oat and rice. QuEChERS extraction was applied obtaining suitable extraction recoveries between 80 and 91%, and good repeatability and reproducibility values. Method Quantification Limits were between 0.05μgL (for aflatoxin G and aflatoxin B) and 15μgL (for deoxynivalenol and fumonisin B). This is the first time that plant-based beverages have been analysed, and certain mycotoxins, such as deoxynivalenol, aflatoxin B, aflatoxin B, aflatoxin G, aflatoxin G, ochratoxin A, T-2 toxin and zearalenone, were found in the analysed samples, and some of them quantified between 0.1μgL and 19μgL.
建立了一种同时测定植物性饮料基质中 11 种真菌毒素的方法，采用 QuEChERS 提取，然后用超高效液相色谱-串联质谱检测（UHPLC-（ESI）MS/MS）。该多真菌毒素方法应用于分析大豆、燕麦和大米等植物性饮料。采用 QuEChERS 提取，获得了 80%至 91%之间的适宜提取回收率，以及良好的重复性和再现性值。方法定量限在 0.05μg/L（黄曲霉毒素 G 和黄曲霉毒素 B）和 15μg/L（脱氧雪腐镰刀菌烯醇和伏马菌素 B）之间。这是首次分析植物性饮料，发现了某些真菌毒素，如脱氧雪腐镰刀菌烯醇、黄曲霉毒素 B、黄曲霉毒素 B、黄曲霉毒素 G、黄曲霉毒素 G、赭曲霉毒素 A、T-2 毒素和玉米赤霉烯酮，并且在分析样品中对其中一些毒素进行了定量，范围在 0.1μg/L 至 19μg/L 之间。
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