基于分子印迹聚合物膜的孔雀石绿仿生酶联免疫吸附测定
Biomimetic ELISA detection of malachite green based on molecularly imprinted polymer film.
作者信息
Li Lu, Peng Ai-Hong, Lin Zheng-Zhong, Zhong Hui-Ping, Chen Xiao-Mei, Huang Zhi-Yong
机构信息
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China.
出版信息
Food Chem. 2017 Aug 15;229:403-408. doi: 10.1016/j.foodchem.2017.02.090. Epub 2017 Feb 21.
A highly selective and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of malachite green (MG) using a molecularly imprinted polymer (MIP) film as bionic antibody. The MIP film, based on the self-polymerization of dopamine, was fabricated on the surfaces of a 96-well microplate. It showed specific recognition for MG in aqueous solution. A direct competitive ELISA method was established with the sensitivity reaching 10.31μgL and the detection limit being 0.3μgL. The cross-reactivity of two structural analogues to MG was less than 10%. The average recovery tested by MG standard spiking was 88.8% for bass and 90.4% for water, and the relative standard deviations were less than 3.6%. All the above results indicated that the developed method could be used to detect MG in fish and water samples rapidly, specifically and accurately.
开发了一种高选择性和灵敏的酶联免疫吸附测定法（ELISA），用于检测孔雀石绿（MG），该方法使用分子印迹聚合物（MIP）膜作为仿生抗体。基于多巴胺的自聚合反应，在96孔微孔板表面制备了MIP膜。该膜对水溶液中的MG表现出特异性识别能力。建立了直接竞争ELISA法，灵敏度达到10.31μg/L，检测限为0.3μg/L。两种与MG结构类似物的交叉反应率小于10%。通过向鲈鱼和水样中添加MG标准品进行回收率测试，平均回收率鲈鱼为88.8%，水为90.4%，相对标准偏差小于3.6%。上述所有结果表明，所开发的方法可用于快速、特异性和准确地检测鱼类和水样中的MG。
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