College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China.
Biosensors (Basel). 2022 Jun 26;12(7):456. doi: 10.3390/bios12070456.
In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T line, blue T line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (20 min, HPLC3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products.
在这项研究中，我们设计了一种带有双检测线（TS-DTL）和双色金纳米探针的快速三色免疫层析测试条，实现了对甲磺酸托烷司琼（TMS）和孔雀石绿（MG）的同时检测。通过一个独特的三色系统（红色 T 线、蓝色 T 线和紫色 C 线），可以在现场快速进行 TMS（0.2 μg/mL）和 MG（0.5 μg/mL）的目视识别，提高了肉眼观察的准确性。TMS 在养殖水、鱼和虾中的 LOD 分别为 11.0、29.6 和 61.4 ng/mL。MG 的 LOD 分别为 47.0 ng/mL（养殖水）、82.8 ng/mL（鱼）和 152.4 ng/mL（虾）。MG 的 LOD 接近类似的 TS 方法。然而，TMS 的目视检测可以满足 TMS 在 USA 残留限量（1 μg/mL）的要求，TMS 的定量检测比 USA 标准低 16 倍以上。该开发平台快速（20 min，HPLC3 h）且准确，经传统 HPLC 方法验证。在三种实际样品中，回收率范围为 82.2%至 108.6%，表明该平台在水产品中 TMS 和 MG 残留的现场快速筛选或多检测方面具有潜在应用。
