Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Yuseong, Daejeon 34113, South Korea.
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, South Korea.
Food Chem. 2017 Aug 15;229:472-478. doi: 10.1016/j.foodchem.2017.02.112. Epub 2017 Feb 24.
Certified reference materials (CRMs; KRISS CRM 108-03-003, 108-03-004) were developed for the accurate determination of fluoroquinolones (enrofloxacin and ciprofloxacin, respectively) in chicken meat. Two groups of chickens were cured with feeds containing enrofloxacin and ciprofloxacin, respectively. After slaughter, the thigh and breast meats were combined for the respective groups and the meat was freeze-dried, pulverized, sieved, and V-mixed. The final bulk material was bottled in 10g portions. For certification of the CRMs, isotope dilution-liquid chromatography/tandem mass spectrometry was used. The certified values of the CRMs were (19.06±0.86)mg/kg for enrofloxacin and (1.095±0.038)mg/kg for ciprofloxacin. The stabilities of the CRMs were monitored at -70°C for 12months, at -20°C for 2months, and at room temperature for 1month. Both CRM candidates were stable during the monitoring period for each temperature.
为了准确测定鸡肉中的氟喹诺酮类药物（恩诺沙星和环丙沙星），开发了认证参考物质（CRMs；KRISS CRM 108-03-003、108-03-004）。两组鸡分别用含有恩诺沙星和环丙沙星的饲料治疗。屠宰后，将大腿肉和鸡胸肉分别混合，并将肉冷冻干燥、粉碎、过筛和 V 型混合。最终的散装材料装在 10g 的小瓶中。为了对 CRMs 进行认证，使用了同位素稀释-液相色谱/串联质谱法。CRMs 的认证值分别为恩诺沙星（19.06±0.86）mg/kg和环丙沙星（1.095±0.038）mg/kg。在 -70°C 下监测 CRMs 的稳定性 12 个月，在 -20°C 下监测 2 个月，在室温下监测 1 个月。在每种温度下的监测期内，两种 CRM 候选物均稳定。
