Sýs Milan, Švecová Blanka, Švancara Ivan, Metelka Radovan
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 532 10, Czech Republic.
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 532 10, Czech Republic.
Food Chem. 2017 Aug 15;229:621-627. doi: 10.1016/j.foodchem.2017.02.068. Epub 2017 Feb 16.
A new electroanalytical method for determination of vitamin E in the form of the total content of tocopherols present in margarines and edible oils has been developed. The method is based on extraction of these biologically active compounds into silicone oil, acting as lipophilic binder of glassy carbon paste electrode, with subsequent electrochemical detection by square wave anodic stripping voltammetry (SWASV) in 0.1M HNO. The values of vitamin E contents were expressed as mass equivalent of α-tocopherol known as the most active form of this lipophilic vitamin. The linear ranges for α-tocopherol determination were 5×10-4×10 and 5×10-1×10molL with the detection limits of 1×10 and 3.3×10molL for 5 and 15min accumulation, respectively. The results have shown that SWASV with extraction step is very sensitive method for the determination of vitamin E, being comparable to reversed-phase high performance liquid chromatography chosen as reference method.
已开发出一种新的电分析方法，用于测定人造黄油和食用油中生育酚总含量形式的维生素E。该方法基于将这些生物活性化合物萃取到硅油中，硅油作为玻碳糊电极的亲脂性粘合剂，随后在0.1M HNO中通过方波阳极溶出伏安法（SWASV）进行电化学检测。维生素E含量的值以α-生育酚的质量当量表示，α-生育酚是这种亲脂性维生素最具活性的形式。α-生育酚测定的线性范围为5×10 - 4×10和5×10 - 1×10mol/L，5分钟和15分钟积累时的检测限分别为1×10和3.3×10mol/L。结果表明，带有萃取步骤的SWASV是测定维生素E的非常灵敏的方法，与作为参考方法的反相高效液相色谱法相当。
