Karmowski Jasmin, Hintze Victoria, Kschonsek Josephine, Killenberg Margrit, Böhm Volker
Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Straße 25-29, D-07743 Jena, Germany.
Analytik Jena AG, Jena, Germany.
Food Chem. 2015 May 15;175:593-600. doi: 10.1016/j.foodchem.2014.12.010. Epub 2014 Dec 11.
The purpose of this study was to measure the antioxidant activity (AOA) of tocopherols and tocotrienols by using photochemiluminescence (PCL). This method enables to detect total lipophilic antioxidants. The AOA of all vitamin E isomers depended on number and position of methyl groups in the chroman ring. Correlation between the AOA and the redox potential and the biological activity of the tocochromanols was observed. The second aim was to analyse different kinds of wheat, vegetable oils, milk and milk cream on their antioxidant capacity (AOC) by using PCL and α-TEAC. The contents of vitamin E and carotenoids were analysed by HPLC. Correlations between the sum of carotenoids and vitamin E and the AOC were detected. Based on high vitamin E contents, the oils had the highest and in contrast, the product macaroni showed the lowest AOC. A concentration-dependent effect was observed in both assays, PCL and α-TEAC.
本研究的目的是通过光化学发光法（PCL）测定生育酚和生育三烯酚的抗氧化活性（AOA）。该方法能够检测总亲脂性抗氧化剂。所有维生素E异构体的AOA取决于色满环中甲基的数量和位置。观察到AOA与生育色满醇的氧化还原电位和生物活性之间的相关性。第二个目的是通过PCL和α-TEAC分析不同种类的小麦、植物油、牛奶和奶油的抗氧化能力（AOC）。通过高效液相色谱法（HPLC）分析维生素E和类胡萝卜素的含量。检测到类胡萝卜素和维生素E的总和与AOC之间的相关性。基于高维生素E含量，油类的AOC最高，相反，通心粉产品的AOC最低。在PCL和α-TEAC这两种测定方法中均观察到浓度依赖性效应。
