Lippitsch Anne, Chukovetskyi Yuri, Baal Nelli, Bein Gregor, Hackstein Holger
Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
Cell Immunol. 2017 Jun;316:41-52. doi: 10.1016/j.cellimm.2017.03.005. Epub 2017 Mar 29.
Plasmacytoid dendritic cells (pDC) are of increasing interest in cancer vaccine development, but many functions of these highly specialized, multifaceted cells are poorly understood. The transferrin receptor CD71 has also been suggested to function as an antigen uptake receptor on professional antigen-presenting cells. In this study, we employed multiparameter flow cytometry to investigate CD71 expression on various leukocyte subsets, including DC subsets, granulocytes, macrophages, T and B lymphocytes, γδ T cells, and natural killer cells. Cells from various lymphoid and non-lymphoid murine tissues were analyzed using fluorochrome-conjugated monoclonal antibodies. High CD71 expression (90-100%) was observed, uniquely on pDC amongst the leukocyte populations examined, in both lymphoid and non-lymphoid tissues, including other DC subsets. In contrast, CD71 expression on non-tissue pDC, in the bone marrow and peripheral blood, was reduced. The cause and function of this high tissue pDC-selective CD71 expression remain to be examined.
浆细胞样树突状细胞（pDC）在癌症疫苗开发中越来越受到关注，但这些高度专业化、多面化细胞的许多功能仍知之甚少。转铁蛋白受体CD71也被认为在专职抗原呈递细胞上作为抗原摄取受体发挥作用。在本研究中，我们采用多参数流式细胞术研究CD71在各种白细胞亚群上的表达，包括树突状细胞亚群、粒细胞、巨噬细胞、T和B淋巴细胞、γδT细胞以及自然杀伤细胞。使用荧光染料偶联的单克隆抗体分析来自各种淋巴和非淋巴小鼠组织的细胞。在包括其他树突状细胞亚群在内的淋巴和非淋巴组织中，在所检查的白细胞群体中，仅在浆细胞样树突状细胞上观察到高CD71表达（90 - 100%）。相比之下，骨髓和外周血中非组织性浆细胞样树突状细胞上的CD71表达降低。这种高组织性浆细胞样树突状细胞选择性CD71表达的原因和功能仍有待研究。
