MOE Key Laboratory of Marine Genetics and Breeding, and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute, Ocean University of China, Qingdao, China.
Hainan Yazhou Bay Seed Laboratory, Sanya, China.
Front Immunol. 2023 Apr 27;14:1190590. doi: 10.3389/fimmu.2023.1190590. eCollection 2023.
The CpG oligodeoxynucleotides (CpG ODNs) reportedly possess the capacity to strengthen immunity in mammals. This experiment was conducted to evaluate the impact of dietary supplementation with 17 types of CpG ODNs on intestinal microbiota diversity, antioxidant capacity, and immune-related gene expression profiles of the shrimp . Diets including 50 mg kg CpG ODNs wrapped in egg whites were prepared and divided into 17 different groups, with 2 control groups (normal feed and feed with egg whites). These CpG ODNs supplemented diets and the control diets were fed to (5.15 ± 0.54 g) three times daily at 5%-8% shrimp body weight for three weeks. The results of consecutive detection of intestinal microbiota by 16S rDNA sequencing indicated that 11 of the 17 types of CpG ODNs significantly enhanced intestinal microbiota diversity, increased the populations of several probiotic bacteria, and activated possible mechanisms relevant to diseases. The immune-related genes expression and antioxidant capacity in hepatopancreas further demonstrated that the 11 types of CpG ODNs effectively improved the innate immunity of shrimp. Additionally, histology results showed that the CpG ODNs in the experiment did not damage the tissue structure of hepatopancreas. The results suggest that CpG ODNs could be used as a trace supplement to improve the intestinal health and immunity of shrimp.
据报道，CpG 寡脱氧核苷酸（CpG ODNs）具有增强哺乳动物免疫力的能力。本实验旨在评估膳食补充 17 种 CpG ODN 对虾肠道微生物多样性、抗氧化能力和免疫相关基因表达谱的影响。制备了包含 50mg/kg 包被在蛋清中的 CpG ODN 的饲料，并分为 17 个不同组，包括 2 个对照组（正常饲料和含蛋清的饲料）。这些 CpG ODN 补充饲料和对照饲料以 5%-8%虾体重的 5.15 ± 0.54g 三次每日喂养 3 周。16S rDNA 测序连续检测肠道微生物群的结果表明，17 种 CpG ODN 中的 11 种显著增强了肠道微生物群的多样性，增加了几种益生菌的种群，并激活了与疾病相关的可能机制。肝胰腺中免疫相关基因的表达和抗氧化能力进一步表明，11 种 CpG ODN 有效改善了虾的先天免疫力。此外，组织学结果表明，实验中的 CpG ODN 未损伤肝胰腺的组织结构。结果表明，CpG ODN 可以作为一种痕量补充剂来改善虾的肠道健康和免疫力。
