Khosrawipour Veria, Khosrawipour Tanja, Hedayat-Pour Yousef, Diaz-Carballo David, Bellendorf Alexander, Böse-Ribeiro Hugo, Mücke Ralph, Mohanaraja Nirushika, Adamietz Irenäus Anton, Fakhrian Khashayar
Department of Neuroanatomy, Ruhr University, Bochum, Germany.
Department of General Surgery, Marien Hospital Herne, Ruhr University, Bochum, Germany.
Anticancer Res. 2017 Apr;37(4):1677-1680. doi: 10.21873/anticanres.11498.
This study was performed to evaluate the impact of whole-abdominal irradiation on local penetration of doxorubicin into the peritoneum and the abdominal organs in a post-mortem swine model.
Doxorubicin was aerosolized into the abdominal cavity of swine at a pressure of 12 mmHg CO at room temperature (25°). One swine was subjected to pressurized intraperitoneal aerosol chemotherapy (PIPAC) using Micropump without irradiation; the second one received 2 Gy and the third one 7 Gy whole-abdominal irradiation, 15 min prior to PIPAC application. Samples of the peritoneal surface were extracted at different positions from within the abdominal cavity. In-tissue doxorubicin penetration was measured using fluorescence microscopy on frozen thin sections.
The depth of penetration of doxorubicin was found to be wide-ranging, between 17 μm on the surface of the stomach and 348 μm in the small intestine. The penetration depth into the small intestine was 348 μm, 312 μm and 265 μm for PIPAC alone, PIPAC with 2 Gy irradiation and PIPAC with 7 Gy irradiation, respectively (p<0.05). The penetration into the liver was 64 μm, 55 μm and 40 μm, respectively (p=0.05).
Irradiation was not found to increase the depth of doxorubicin penetration into normal tissue in the post-mortem swine model. A reduction of doxorubicin penetration was observed after application of higher irradiation doses. Further studies are warranted to determine if irradiation can be used safely as chemopotentiating agent for patients with peritoneal metastases treated with PIPAC.
本研究旨在评估在猪死后模型中，全腹照射对阿霉素在腹膜和腹部器官局部渗透的影响。
在室温（25°）下，以12 mmHg二氧化碳压力将阿霉素雾化至猪腹腔内。一头猪在未照射的情况下使用微量泵进行加压腹腔内气溶胶化疗（PIPAC）；第二头猪在PIPAC应用前15分钟接受2 Gy全腹照射；第三头猪接受7 Gy全腹照射。从腹腔内不同位置提取腹膜表面样本。使用荧光显微镜对冷冻薄切片测量组织内阿霉素的渗透情况。
发现阿霉素的渗透深度范围广泛，在胃表面为17μm，在小肠中为348μm。单独使用PIPAC、PIPAC联合2 Gy照射和PIPAC联合7 Gy照射时，阿霉素在小肠中的渗透深度分别为348μm、312μm和265μm（p<0.05）。在肝脏中的渗透深度分别为64μm、55μm和40μm（p=0.05）。
在猪死后模型中，未发现照射会增加阿霉素在正常组织中的渗透深度。在应用较高照射剂量后，观察到阿霉素渗透减少。有必要进一步研究以确定照射是否可安全用作接受PIPAC治疗的腹膜转移患者的化学增效剂。
