Lee Doyun, Lee Albert K
Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34141, Republic of Korea;
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147
Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot095802. doi: 10.1101/pdb.prot095802.
Whole-cell recording has been used to measure and manipulate a neuron's spiking and subthreshold membrane potential, allowing assessment of the cell's inputs and outputs as well as its intrinsic membrane properties. This technique has also been combined with pharmacology and optogenetics as well as morphological reconstruction to address critical questions concerning neuronal integration, plasticity, and connectivity. This protocol describes a technique for obtaining whole-cell recordings in awake head-fixed animals, allowing such questions to be investigated within the context of an intact network and natural behavioral states. First, animals are habituated to sit quietly with their heads fixed in place. Then, a whole-cell recording is obtained using an efficient, blind patching protocol. We have successfully applied this technique to rats and mice.
全细胞记录已被用于测量和操纵神经元的放电以及阈下膜电位，从而能够评估细胞的输入和输出以及其内在膜特性。该技术还与药理学、光遗传学以及形态学重建相结合，以解决有关神经元整合、可塑性和连接性的关键问题。本方案描述了一种在清醒的头部固定动物中获得全细胞记录的技术，使得这些问题能够在完整的神经网络和自然行为状态的背景下进行研究。首先，让动物适应头部固定在位并安静地坐着。然后，使用一种高效的盲插膜片钳记录方案获得全细胞记录。我们已成功地将该技术应用于大鼠和小鼠。
