Margrie Troy W, Brecht Michael, Sakmann Bert
Abteilung Zellphysiologie, Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, Heidelberg 69120, Germany.
Pflugers Arch. 2002 Jul;444(4):491-8. doi: 10.1007/s00424-002-0831-z. Epub 2002 Apr 17.
A blind patch-clamp technique for in vivo whole-cell recordings in the intact brain is described. Recordings were obtained from various neuronal cell types located 100-5,000 microm from the cortical surface. Access resistance of recordings was as low as 10 M Omega but increased with recording depth and animal age. Recordings were remarkably stable and it was therefore possible to obtain whole-cell recordings in awake, head-fixed animals. The whole-cell configuration permitted rapid dialysis of cells with a calcium buffer. In most neurons very little ongoing action potential (AP) activity was observed and the spontaneous firing rates were up to 50-fold less than what has been reported by extracellular unit recordings. AP firing in the brain might therefore be far sparser than previously thought.
本文描述了一种用于在完整大脑中进行体内全细胞记录的盲法膜片钳技术。记录来自距皮质表面100 - 5000微米处的各种神经元细胞类型。记录的接入电阻低至10兆欧，但随记录深度和动物年龄增加。记录非常稳定，因此可以在清醒、头部固定的动物中获得全细胞记录。全细胞配置允许用钙缓冲液对细胞进行快速透析。在大多数神经元中，观察到的持续动作电位（AP）活动极少，自发放电率比细胞外单位记录所报道的低50倍。因此，大脑中的AP放电可能比以前认为的要稀疏得多。
