Zhang Chao, Chi Yudan, Zhou Dongming
Vaccine Research Center, Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, University of Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, China.
Methods Mol Biol. 2017;1581:3-13. doi: 10.1007/978-1-4939-6869-5_1.
Vaccination is considered to be the most effective method of preventing infectious or other diseases. Adenovirus (Ad) is one the most promising vectors in vaccine research and development. It can induce not only potent humoral but also cellular immune responses, and has therefore been widely applied in basic and translational studies. Chimpanzee Ad is a rare serotype circulating in humans. This circumvents the problem of preexisting immunity to human Ad serotypes, enhancing Chimpanzee Ad prospects in vaccine development. Here we describe experimental procedures used to generate a new generation of rabies vaccine based on a chimpanzee Ad vector, which can be extended in the development of novel vaccines against other infectious diseases.
接种疫苗被认为是预防传染病或其他疾病的最有效方法。腺病毒（Ad）是疫苗研发中最有前景的载体之一。它不仅能诱导强大的体液免疫反应，还能诱导细胞免疫反应，因此已广泛应用于基础研究和转化研究。黑猩猩腺病毒是在人类中传播的一种罕见血清型。这避免了对人类腺病毒血清型预先存在免疫的问题，增强了黑猩猩腺病毒在疫苗开发中的前景。在此，我们描述了用于基于黑猩猩腺病毒载体生产新一代狂犬病疫苗的实验程序，该程序可扩展用于开发针对其他传染病的新型疫苗。
