Roper Rachel L
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
Methods Mol Biol. 2017;1581:121-129. doi: 10.1007/978-1-4939-6869-5_7.
Poxviruses cause many diseases in humans and animals worldwide, and there is a need for vaccines with improved safety and good efficacy. In addition, poxvirus vectors are widely used as recombinant vaccines for various infectious diseases and as recombinant and oncolytic vaccines for cancer. One concern with poxvirus vaccine vectors is that some poxviruses can infect a developing fetus and cause fetal loss or congenital disease. This can be an issue both for patients receiving a vaccine and for pregnant health care providers, including doctors, nurses, and veterinarians, who might receive accidental exposure to the poxvirus by injection or during patient care. We describe here a method for analyzing the safety of virus exposure in pregnant mammals using a mouse model testing vaccinia, canarypox, and raccoonpox virus vectors.
痘病毒在全球范围内导致人类和动物的多种疾病，因此需要安全性更高且疗效良好的疫苗。此外，痘病毒载体被广泛用作针对各种传染病的重组疫苗以及针对癌症的重组和溶瘤疫苗。对痘病毒疫苗载体的一个担忧是，一些痘病毒可感染发育中的胎儿并导致流产或先天性疾病。这对于接受疫苗接种的患者以及包括医生、护士和兽医在内的怀孕医护人员而言都可能是个问题，他们可能在注射疫苗或护理患者过程中意外接触到痘病毒。我们在此描述一种利用小鼠模型检测痘苗病毒、金丝雀痘病毒和浣熊痘病毒载体来分析怀孕哺乳动物病毒暴露安全性的方法。
