Lagarde A E, Franchi A J, Paris S, Pouysségur J M
Mount Sinai Hospital Research Institute, Division of Cancer and Cell Biology, Toronto, Ontario, Canada.
J Cell Biochem. 1988 Mar;36(3):249-60. doi: 10.1002/jcb.240360306.
Mutants unable to regulate intracellular pH through the Na+: H+ antiport system were found to evolve tumors less frequently than wild-type CCL39 hamster lung fibroblasts, after transplantation in athymic nude mice. When rare tumors arose, they comprised cells which were transformed in vitro, but which upon retransplantation grew at a lower rate than tumor cells originating from CCL39 cells. Both parental and mutant cells became transformed after transfection of the activated Harvey ras oncogene, but transfectants derived from the mutants had a weaker tumorigenic potential. These results suggest that transformed characteristics can be acquired independently from the Na+: H+ antiporter. However, the presence of this system provides a selective growth advantage when cells are confronted with natural environments, as it occurs during the expansion of tumors in a host.
在无胸腺裸鼠体内移植后，发现无法通过Na⁺:H⁺反向转运系统调节细胞内pH值的突变体比野生型CCL39仓鼠肺成纤维细胞发生肿瘤的频率更低。当罕见肿瘤出现时，它们包含在体外已转化的细胞，但再次移植后，其生长速度低于源自CCL39细胞的肿瘤细胞。亲本细胞和突变体细胞在转染活化的哈维鼠肉瘤病毒癌基因后均发生转化，但源自突变体的转染子具有较弱的致瘤潜力。这些结果表明，转化特征可以独立于Na⁺:H⁺反向转运体获得。然而，当细胞面对自然环境时，如在宿主肿瘤生长过程中，该系统的存在提供了选择性生长优势。
