Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland; email:
Annu Rev Anal Chem (Palo Alto Calif). 2017 Jun 12;10(1):1-24. doi: 10.1146/annurev-anchem-061516-045219. Epub 2017 Mar 24.
Recent years have witnessed an increased use of droplet-based microfluidic techniques in a wide variety of chemical and biological assays. Nevertheless, obtaining dynamic data from these platforms has remained challenging, as this often requires reading the same droplets (possibly thousands of them) multiple times over a wide range of intervals (from milliseconds to hours). In this review, we introduce the elemental techniques for the formation and manipulation of microfluidic droplets, together with the most recent developments in these areas. We then discuss a wide range of analytical methods that have been successfully adapted for analyte detection in droplets. Finally, we highlight a diversity of studies where droplet-based microfluidic strategies have enabled the characterization of dynamic systems that would otherwise have remained unexplorable.
近年来，基于液滴的微流控技术在各种化学和生物分析中得到了越来越多的应用。然而，从这些平台获取动态数据仍然具有挑战性，因为这通常需要多次读取相同的液滴（可能成千上万），并且时间间隔范围很宽（从毫秒到小时）。在这篇综述中，我们介绍了形成和操纵微流控液滴的基本技术，以及这些领域的最新进展。然后，我们讨论了一系列已成功适用于液滴中分析物检测的分析方法。最后，我们强调了许多研究，其中基于液滴的微流控策略使对动态系统的特征描述成为可能，否则这些系统将无法探索。
