菊欧文氏菌B374菌株中pel和cel基因的染色体定位

Chromosomal mapping of the pel and cel genes in Erwinia chrysanthemi strain B374.

作者信息

Piecq M, van Gijsegem F, Schoonejans E, Toussaint A

机构信息

Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.

出版信息

Mol Microbiol. 1988 Mar;2(2):297-302. doi: 10.1111/j.1365-2958.1988.tb00032.x.

DOI:10.1111/j.1365-2958.1988.tb00032.x

PMID:2837618

Abstract

Using the RP4::mini-Mu in vivo cloning technique, van Gijsegem et al. (1985) isolated several pel and cel genes of Erwinia chrysanthemi (Ech) B374 strain. We have localized these genes on the Ech chromosome by co-transfer mapping of MudI1734 insertion mutants and refined the map by co-transposition analysis. This analysis has enabled us to identify another cel gene.

摘要

使用RP4::mini-Mu体内克隆技术，范·吉斯格姆等人（1985年）分离出了菊欧文氏菌（Ech）B374菌株的几个pel和cel基因。我们通过MudI1734插入突变体的共转移定位将这些基因定位在Ech染色体上，并通过共转座分析完善了图谱。该分析使我们能够鉴定出另一个cel基因。