van Gijsegem F
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
Mol Microbiol. 1989 Oct;3(10):1415-24. doi: 10.1111/j.1365-2958.1989.tb00124.x.
In this paper, we have used filter hybridization and nucleotide sequencing to analyse the relationship between the three genes of the pelADE cluster in the Erwinia chrysanthemi (Ech) strain B374. This cluster encodes for three of the five pectate lyase proteins that are involved in the maceration and soft-rotting of plant tissue, an important trait in Ech pathogenicity. Southern hybridization revealed homology between each of the three pel genes. A 3560 bp DNA fragment containing the pelE and pelD genes was sequenced. These two genes show extensive homology in the coding regions but only low homology in the 5' and 3' non-coding regions. However both genes exhibit sequences homologous to the Escherichia coli CAP-binding site consensus sequence upstream of the start codon and an inverted repeat sequence which may act as a rho-independent transcriptional terminator after the translational stop. The pel genes of Ech B374 were also compared with the already sequenced pel genes of EC16, another Ech strain.
在本文中，我们利用滤膜杂交和核苷酸测序技术分析了菊欧文氏菌（Ech）B374菌株中pelADE基因簇的三个基因之间的关系。该基因簇编码五种果胶酸裂解酶蛋白中的三种，这些蛋白参与植物组织的浸解和软腐，这是Ech致病性的一个重要特征。Southern杂交揭示了三个pel基因之间的同源性。对包含pelE和pelD基因的3560 bp DNA片段进行了测序。这两个基因在编码区显示出广泛的同源性，但在5'和3'非编码区仅显示出低同源性。然而，两个基因在起始密码子上游均表现出与大肠杆菌CAP结合位点共有序列同源的序列，以及一个反向重复序列，该序列在翻译终止后可能作为不依赖ρ的转录终止子。还将Ech B374的pel基因与另一个Ech菌株EC16的已测序pel基因进行了比较。
