He Y N, Zhang S C, Zhang H M
JOINN Laboratories, Beijing 100176, China.
Zhonghua Bing Li Xue Za Zhi. 2017 Apr 8;46(4):249-254. doi: 10.3760/cma.j.issn.0529-5807.2017.04.007.
To investigate the spontaneous neoplastic lesions and their incidences in Sprague-Dawley (SD) and Wistar rats, and to accumulate background data for carcinogenicity studies. One hundred and eighty SD rats and 240 Wistar rats (4-week old) , half in each sex, were used in this study. The rats were housed routinely under specific pathogen-free environment and euthanized after 104 weeks. Histopathological examination was undertaken for all animals including deaths and scheduled euthanasia. The types and incidences of spontaneous tumors were gathered statistically. Total 411 rats (176 SD rats and 235 Wistar rats) were examined in this study. The total tumor incidence of the 411 rats was 57.7%(237/411). The total tumor incidence in SD rats was 55.7%(98/176), benign tumor incidence was 48.9%(86/176) and malignant tumor incidence was 15.9%(28/176). The total tumor incidence in Wistar rats was 59.1%(139/235), benign tumor incidence was 51.5%(121/235) and malignant tumor incidence was 14.5%(34/235). The main benign tumors were pituitary adenoma (23.3% in SD rats, 12.3% in Wistar rats), breast fibroadenoma (21.3% in SD rats, 12.9% in Wistar rats) and breast adenoma (16.9% in SD rats, 9.5% in Wistar rats) in females; testis Leydig cell tumor (0 in SD rats, 14.3% in Wistar rats) in males. The main malignant tumors were breast carcinoma (10.1% in SD rats, 3.4% in Wistar rats) and uterine leiomyosarcoma (0 in SD rats, 2.6% in Wistar rats) in females; squamous cell carcinoma of skin (2.3% in SD rats, 0.9% in Wistar rats); subcutaneous fibrosarcoma (1.1% in SD rats, 2.1% in Wistar rats); brain malignant glioma (1.1% in SD rats, 1.7% in Wistar rats). In the study, a high incidence of spontaneous tumors is reported in both SD and Wistar rats housed for 2 years. The incidence of benign tumors is higher than that of malignant rumors. The benign tumors mainly are pituitary adenoma, breast fibroadenoma and breast adenoma in females, and testis Leydig cell tumor in males. The malignant tumors mainly are breast carcinoma and some soft tissue sarcomas. The results of the study enrich the data of spontaneous tumor in SD and Wisar rats and provide background data for carcinogenicity studies for new drugs.
为研究斯普拉格-道利（SD）大鼠和Wistar大鼠的自发性肿瘤病变及其发生率，并积累致癌性研究的背景数据。本研究使用了180只SD大鼠和240只Wistar大鼠（4周龄），每种性别各半。大鼠常规饲养于无特定病原体环境中，104周后安乐死。对所有动物包括死亡和计划安乐死的动物进行组织病理学检查。对自发性肿瘤的类型和发生率进行统计学汇总。本研究共检查了411只大鼠（176只SD大鼠和235只Wistar大鼠）。411只大鼠的总肿瘤发生率为57.7%（237/411）。SD大鼠的总肿瘤发生率为55.7%（98/176），良性肿瘤发生率为48.9%（86/176），恶性肿瘤发生率为15.9%（28/176）。Wistar大鼠的总肿瘤发生率为59.1%（139/235），良性肿瘤发生率为51.5%（121/235），恶性肿瘤发生率为14.5%（34/235）。雌性主要的良性肿瘤为垂体腺瘤（SD大鼠中为23.3%，Wistar大鼠中为12.3%）、乳腺纤维腺瘤（SD大鼠中为21.3%，Wistar大鼠中为12.9%）和乳腺腺瘤（SD大鼠中为16.9%，Wistar大鼠中为9.5%）；雄性为睾丸间质细胞瘤（SD大鼠中为0，Wistar大鼠中为14.3%）。雌性主要的恶性肿瘤为乳腺癌（SD大鼠中为10.1%，Wistar大鼠中为3.4%）和子宫平滑肌肉瘤（SD大鼠中为0，Wistar大鼠中为2.6%）；皮肤鳞状细胞癌（SD大鼠中为2.3%，Wistar大鼠中为0.9%）；皮下纤维肉瘤（SD大鼠中为1.1%，Wistar大鼠中为2.1%）；脑恶性胶质瘤（SD大鼠中为1.1%，Wistar大鼠中为1.7%）。在本研究中，报告了饲养两年的SD大鼠和Wistar大鼠均有较高的自发性肿瘤发生率。良性肿瘤的发生率高于恶性肿瘤。良性肿瘤主要为雌性的垂体腺瘤、乳腺纤维腺瘤和乳腺腺瘤，以及雄性的睾丸间质细胞瘤。恶性肿瘤主要为乳腺癌和一些软组织肉瘤。本研究结果丰富了SD大鼠和Wistar大鼠自发性肿瘤的数据，并为新药致癌性研究提供了背景数据。
