Said Azza Mohamed Ahmed, Zaki Rania Gamal Eldin, Salah Eldin Rania A, Nasr Maha, Azab Samar Saad, Elzankalony Yaser Abdelmageuid
Ophthalmology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
, 10th Fawzy Elmoteay Street, Heliopolis, Cairo, 11361, Egypt.
BMC Ophthalmol. 2017 Apr 4;17(1):38. doi: 10.1186/s12886-017-0433-3.
Retinopathy of prematurity (ROP) is one of the targets for early detection and treatment to prevent childhood blindness in world health organization programs. The purpose of study was to evaluate the efficacy of intravitreal injection of 2-Methoxyestradiol (2-ME) nanoemulsion in regressing neovascularization of a ROP rat model.
A prospective comparative case - control animal study conducted on 56 eyes of 28 healthy new born Sprague Dawley male albino rat. ROP was induced in 21 rats then two concentrations of 2-ME nanoparticles were injected in right eyes of 14 rats (low dose; study group I, high dose; study group II). A blank nanoemulsion was injected in the right eyes of seven rats (control positive group I). No injections performed in contralateral left eyes (control positive group II). Seven rats (14 eyes) were kept in room air (control negative group). On postnatal day 17, eyeballs were enucleated. Histological structure of the retina was examined using Hematoxylin and eosin staining. Vascular endothelial growth factor (VEGF) and glial fibrillary acidic protein (GFAP) expressions were detected by immunohistochemical studies.
Intravitreal injection of 2-ME (in the two concentrations) caused marked regression of the new vascular tufts on the vitreal side with normal organization and thickness of the retina especially in study group II, which also show negative VEGF immunoreaction. Positive GFAP expression was detected in the control positive groups and study group (I).
Intravitreal injection of 2-Methoxyestradiol nanoemulsion is a promising effective method in reduction of neovascularization of a ROP rat model.
早产儿视网膜病变（ROP）是世界卫生组织预防儿童失明项目中早期检测和治疗的目标之一。本研究的目的是评估玻璃体内注射2-甲氧基雌二醇（2-ME）纳米乳剂对ROP大鼠模型新生血管消退的疗效。
对28只健康新生Sprague Dawley雄性白化大鼠的56只眼睛进行前瞻性比较病例对照动物研究。诱导21只大鼠发生ROP，然后对14只大鼠的右眼注射两种浓度的2-ME纳米颗粒（低剂量；研究组I，高剂量；研究组II）。对7只大鼠的右眼注射空白纳米乳剂（对照阳性组I）。对侧左眼不进行注射（对照阳性组II）。将7只大鼠（14只眼睛）置于室内空气中（对照阴性组）。在出生后第17天摘除眼球。使用苏木精和伊红染色检查视网膜的组织结构。通过免疫组织化学研究检测血管内皮生长因子（VEGF）和胶质纤维酸性蛋白（GFAP）的表达。
玻璃体内注射2-ME（两种浓度）导致玻璃体侧新生血管丛明显消退，视网膜组织结构和厚度正常，尤其是在研究组II中，该组还显示VEGF免疫反应阴性。在对照阳性组和研究组（I）中检测到GFAP阳性表达。
玻璃体内注射2-甲氧基雌二醇纳米乳剂是减少ROP大鼠模型新生血管形成的一种有前景的有效方法。
