Akaike N, Kaneda M, Hori N, Krishtal O A
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Neurosci Lett. 1988 Apr 22;87(1-2):75-9. doi: 10.1016/0304-3940(88)90148-6.
The responses to excitatory and inhibitory amino acids have been investigated in isolated pyramidal cells from young and adult rat hippocampus using internal perfusion and 'concentration-clamp' techniques. The neurons dissociated in a purely mechanical way were sensitive to all excitatory amino acids (glutamate, kainate, quisqualate and N-methyl-D-aspartate (NMDA] and inhibitory amino acids (glycine, taurine and gamma-aminobutyric acid). The NMDA response was dramatically potentiated by adding glycine at threshold concentration (10(-6) M). The enzyme treatment of hippocampal slices selectively removed the NMDA sensitivity but did not alter all other pharmacological properties of voltage- and agonist-gated ion channels.
利用内部灌注和“浓度钳制”技术，对来自幼年和成年大鼠海马体的离体锥体细胞对兴奋性和抑制性氨基酸的反应进行了研究。以纯机械方式解离的神经元对所有兴奋性氨基酸（谷氨酸、海人藻酸、quisqualate和N-甲基-D-天冬氨酸（NMDA））以及抑制性氨基酸（甘氨酸、牛磺酸和γ-氨基丁酸）敏感。通过添加阈值浓度（10⁻⁶ M）的甘氨酸，NMDA反应显著增强。对海马体切片进行酶处理可选择性地消除NMDA敏感性，但不会改变电压门控和激动剂门控离子通道的所有其他药理学特性。
