Komabayashi T, Izawa T, Nakamura T, Suda K, Shinoda S, Tsuboi M
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
Res Commun Chem Pathol Pharmacol. 1988 Apr;60(1):137-40.
The effects of dibutyryl cyclic AMP (DBcAMP) and cyclic GMP (DBcGMP) on the Na+,K+-ATPase activity and the release of sialic acid were investigated in dog submandibular gland pieces. The Na+,K+-ATPase activity and the release of sialic acid were significantly stimulated in the presence of 100 microM DBcAMP or in the combination of 3 mM NaF and 50 microM 1-methyl-3-isobutylxanthin but were not by DBcGMP. When the pieces were incubated in the Ca2+-free medium, the DBcAMP-induced Na+,K+-ATPase activity slightly decreased but the sialic acid release was unaffected. The corresponding results were also observed in the Ca2+-free medium supplemented with 2 mM EGTA. These results suggest that the cytosolic cyclic AMP modifies the Na+ pump activity.
在犬下颌下腺组织块中研究了二丁酰环磷腺苷（DBcAMP）和环磷鸟苷（DBcGMP）对Na⁺，K⁺-ATP酶活性及唾液酸释放的影响。在存在100微摩尔DBcAMP时，或在3毫摩尔氟化钠与50微摩尔1-甲基-3-异丁基黄嘌呤联合存在时，Na⁺，K⁺-ATP酶活性及唾液酸释放均受到显著刺激，但DBcGMP无此作用。当组织块在无钙培养基中孵育时，DBcAMP诱导的Na⁺，K⁺-ATP酶活性略有下降，但唾液酸释放不受影响。在补充有2毫摩尔乙二醇双乙醚二胺四乙酸（EGTA）的无钙培养基中也观察到了相应结果。这些结果表明，胞质环磷腺苷可改变Na⁺泵活性。
