Berio Enrica, Divari Sara, Starvaggi Cucuzza Laura, Biolatti Bartolomeo, Cannizzo Francesca Tiziana
Department of Veterinary Science, University of Turin , Grugliasco , Torino , Italy.
PeerJ. 2017 Mar 30;5:e3124. doi: 10.7717/peerj.3124. eCollection 2017.
The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17-estradiol.
The skeletal muscle cell line C2C12 was treated with 17-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA levels of myogenic regulatory factors, myosin heavy chain, oxytocin, oxytocin receptor and adipogenic factors were analysed in C2C12 myotubes.
It was demonstrated that C2C12 myoblasts and myotubes express oxytocin and its receptor, in particular the receptor levels physiologically increase in differentiated myotubes. Myotubes treated with 17-estradiol overexpressed oxytocin and oxytocin receptor genes by approximately 3- and 29-fold, respectively. A decrease in the expression of fatty acid binding protein 4 (0.62-fold), a fat metabolism-associated gene, was observed in oxytocin-treated myotubes. On the contrary, fatty acid binding protein 4 was upregulated (2.66-fold) after the administration of the combination of 17-estradiol and oxytocin. 17-estradiol regulates oxytocin and its receptor in skeletal muscle cells and they act in a synergic way on fatty acid metabolism.
Oxytocin and its receptor are physiologically regulated along differentiation. 17-estradiol regulates oxytocin and its receptor in skeletal muscle cells. 17-estradiol and oxytocin act in a synergic way on fatty acid metabolism. A better understanding of the regulation of skeletal muscle homeostasis by estrogens and oxytocin peptide could contribute to increase our knowledge of muscle and its metabolism.
骨骼肌的内分泌学非常复杂，许多关于激素在骨骼肌中作用的问题仍未得到解决。这项工作的目的是增进我们对骨骼肌与17-β-雌二醇之间关系的了解。
用17-β-雌二醇、催产素肽以及这两种激素的组合处理骨骼肌细胞系C2C12。分析C2C12肌管中肌源性调节因子、肌球蛋白重链、催产素、催产素受体和成脂因子的mRNA水平。
结果表明，C2C12成肌细胞和肌管表达催产素及其受体，尤其是在分化的肌管中，受体水平在生理上会增加。用17-β-雌二醇处理的肌管中，催产素和催产素受体基因分别过表达约3倍和29倍。在经催产素处理的肌管中，观察到脂肪代谢相关基因脂肪酸结合蛋白4的表达下降（0.62倍）。相反，在给予17-β-雌二醇和催产素的组合后，脂肪酸结合蛋白4上调（2.66倍）。17-β-雌二醇调节骨骼肌细胞中的催产素及其受体，它们在脂肪酸代谢方面协同发挥作用。
催产素及其受体在分化过程中受到生理调节。17-β-雌二醇调节骨骼肌细胞中的催产素及其受体。17-β-雌二醇和催产素在脂肪酸代谢方面协同发挥作用。更好地了解雌激素和催产素肽对骨骼肌稳态的调节可能有助于增进我们对肌肉及其代谢的认识。
