Wang J, Mi F H, Su X Q, Li Y C, Deng J S, Li S D, Xu D X, Shen Y, Gu X Z
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1987 Sep;9(5):339-41, 18.
A human colonic cancer specimen cut into 1-2 mm3 pieces under aseptic condition was heterotransplanted hypodermically by trocar to the inguinal region of BALB/cATcL nude mice bred in our laboratory. 53 days later, a 10 X 9 X 11 mm3 tumor was obtained. The take rate was 100% (48/48). The take rate of liquid nitrogen frozen and recovered tissue pieces was also 100% (13/13). Grossly, the tumor was rich in blood supply and well encapsulated. Within the tumor, the tissue was cream-colored. G-banded chromosome analysis revealed a human chromosome pattern with the mode 70-90. Pathologic diagnosis was signet-ring cell cancer, which was identical to the surgical specimen. No change was found after maintaining 13 passages in nude mice. It was named the human colonic carcinoma xenograft XHCn/w. It provides an ideal tumor model for in vivo or in vitro experiments.
在无菌条件下，将一份人类结肠癌标本切成1-2立方毫米的小块，通过套管针皮下移植到在本实验室饲养的BALB/cATcL裸鼠的腹股沟区域。53天后，获得了一个10×9×11立方毫米的肿瘤。接种成功率为100%（48/48）。液氮冷冻并复苏的组织块接种成功率也为100%（13/13）。大体上，肿瘤血供丰富，包膜完整。肿瘤内部组织呈淡黄色。G带染色体分析显示人类染色体模式，众数为70-90。病理诊断为印戒细胞癌，与手术标本一致。在裸鼠中传代13次后未发现变化。它被命名为人类结肠癌异种移植瘤XHCn/w。它为体内或体外实验提供了理想的肿瘤模型。
