Structural organization of satellite I chromatin of calf liver.

The organization of liver calf satellite DNA I chromatin has been studied. Precisely seven nucleosomes per DNA satellite repeat (1.4 kb) are shown to be present. Several strict positionings of the nucleosomes along the satellite DNA repeat have been described. One of these positionings, the major phase, was shown to be preferentially organized in clustered repeats. Most of the non-methylated CpG restriction sites were shown to be located in the linker DNAs of the satellite I nucleosomes, whilst, accordingly, methylated HpaII sites were found in the core satellite I DNA. The significance of these results is discussed.