Omori A, Igo-Kemenes T, Zachau H G
Nucleic Acids Res. 1980 Nov 25;8(22):5363-75. doi: 10.1093/nar/8.22.5363.
The nucleosome repeat structure of a rat liver chromatin component containing the satellite I DNA (repeat length 370 bp) was investigated. Digestion experiments with micrococcal nuclease, DNAase II, and the Ca2+/Mg2+-dependent endogenous nuclease of rat liver nuclei revealed a repeat unit of 185 nucleotide pairs which is shorter by approximately 10 bp than the repeat unit of the bulk chromatin of this cell type. The difference seems not to be related to the histone composition which was found to be similar in the two types of chromatin.
对含有卫星I DNA(重复长度370 bp)的大鼠肝脏染色质组分的核小体重复结构进行了研究。用微球菌核酸酶、DNA酶II以及大鼠肝细胞核中依赖Ca2+/Mg2+的内源性核酸酶进行的消化实验表明,存在一个185个核苷酸对的重复单元,该重复单元比这种细胞类型的整体染色质的重复单元短约10 bp。这种差异似乎与组蛋白组成无关,已发现这两种染色质中的组蛋白组成相似。