猪血浆凝溶胶蛋白与 Mg2+ - G - 肌动蛋白在 ATP 和 ADP 存在下形成的二元复合物。

The binary complex of pig plasma gelsolin with Mg2+-G-actin in ATP and ADP.

作者信息

Harris H E

机构信息

AFRC Institute of Animal Physiology and Genetics Research, Cambridge, England.

出版信息

FEBS Lett. 1988 Jun 20;233(2):359-62. doi: 10.1016/0014-5793(88)80460-5.

DOI:10.1016/0014-5793(88)80460-5

PMID:2838335

Abstract

Pig plasma gelsolin combined with Mg-G-actin at less than 10(-8) M Ca2+ to yield a binary complex. Complexes formed from G-actin with bound ATP or ADP. They contained approx. 1 mol of non-exchangeable nucleotide per mol of actin. ATP hydrolysis was not coupled to binary complex formation, but ATP in the complex hydrolysed very slowly. The nucleotide in the binary complex behaved like one of the two nucleotide molecules in the ternary complex (two actin monomers to one gelsolin), but the actin-gelsolin interaction was weaker in the binary complex.

摘要

猪血浆凝溶胶蛋白在钙离子浓度低于10⁻⁸ M时与Mg-G-肌动蛋白结合，形成二元复合物。复合物由结合有ATP或ADP的G-肌动蛋白形成。每摩尔肌动蛋白约含1摩尔不可交换核苷酸。ATP水解与二元复合物的形成不偶联，但复合物中的ATP水解非常缓慢。二元复合物中的核苷酸表现得类似于三元复合物（两个肌动蛋白单体对一个凝溶胶蛋白）中的两个核苷酸分子之一，但二元复合物中肌动蛋白-凝溶胶蛋白的相互作用较弱。