Malta Cristiana P, Damasceno Naiana Nl, Ribeiro Rosangela A, Silva Carolina Sf, Devito Karina L
Schoolof Dentistry, Federal University of Juiz de Fora, Brazil.
Biological Sciences Institute, Federal University of Juiz de Fora, Brazil.
Acta Odontol Latinoam. 2016 Dec;29(3):239-247.
The aim of this study was to evaluate the contamination rate of intra and extraoral digital X ray equipment in a dental radiology clinic at a public educational institution. Samples were collected on three different days, at two times in the day: in the morning, before attending patients, and at the end of the day, after appointment hours and before cleaning and disinfection procedures. Samples were collected from the periapical X-ray machine (tube head, positioning device, control panel and activator button), the panoramic X- ray machine (temporal support, bite block, control panel and activator button), the intraoral digital system (sensor), and the digital system computers (keyboard and mouse). The samples were seeded in different culture media, incubated, and colony forming units (CFU/mL) counted. Biochemical tests were performed for suspected colonies of Staphylococcus, Streptococcus and Gramnegative bacilli (GNB). Fungi were visually differentiated into filamentous fungi and yeasts. The results indicated the growth of fungi and Staphylococcus fromall sampling locations. GNB growth was observed from all sites sampled from the intraoral X-ray equipment. On the panoramic unit, GNB growth was observed in samples from activator button, keyboard and mouse. In general, a higher number of CFU/mL was present before use. It can be concluded that more stringent protocols are needed to control infection and prevent X-ray exams from acting as vehicle for cross contamination.
本研究的目的是评估一所公立教育机构牙科放射科诊所内口腔内和口腔外数字X射线设备的污染率。在三个不同的日子里，于一天中的两个时段采集样本：上午，在接待患者之前；以及一天结束时，在预约时间结束后且在清洁和消毒程序之前。样本取自根尖周X射线机（管头、定位装置、控制面板和激活按钮）、全景X射线机（颞部支撑、咬块、控制面板和激活按钮）、口腔内数字系统（传感器）以及数字系统计算机（键盘和鼠标）。将样本接种于不同的培养基中，进行培养，并对菌落形成单位（CFU/mL）进行计数。对疑似葡萄球菌、链球菌和革兰氏阴性杆菌（GNB）的菌落进行生化试验。真菌在视觉上被区分为丝状真菌和酵母菌。结果表明，在所有采样部位均有真菌和葡萄球菌生长。在口腔内X射线设备的所有采样部位均观察到GNB生长。在全景设备上，在激活按钮、键盘和鼠标的样本中观察到GNB生长。一般来说，使用前的CFU/mL数量更高。可以得出结论，需要更严格的方案来控制感染，并防止X射线检查成为交叉污染的媒介。
