Kalathingal Sajitha, Youngpeter Alison, Minton Jason, Shrout Michael, Dickinson Douglas, Plummer Kevin, Looney Stephen
School of Dentistry, Medical College of Georgia, Augusta, Georgia, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Mar;109(3):457-62. doi: 10.1016/j.tripleo.2009.09.035. Epub 2010 Jan 8.
This study was performed to determine: 1) the rate and source of microbiologic contamination of photostimulable phosphor plates (PSP) in a predoctoral clinic; and 2) whether a combination of weekly gas sterilization and barrier protocols can prevent contamination of PSP plates.
Fifty plates in clinical use and 25 gas-sterilized control plates were selected for examination. The PSP plates were pressed onto blood agar medium and incubated at 37 degrees C. The number, size, distribution, and variety of resulting colonies were noted. To test whether these bacteria could have come from oral sources, 17 colonies were selected for culture on Mitis-Salivarius (M-S) agar. Those colonies that grew on the M-S agar were Gram stained.
Twenty-eight test plates (56%) exhibited growth of bacterial colonies on blood agar. Seventeen of those bacterial colonies were selected for growth on M-S agar, and 13 (76.47%) showed growth, 69% of them gram positive.
Our results indicate reinforcing standard precautions of infection control for intraoral radiography, and that gas sterilization of plates after each day's clinical use is a potential solution.
本研究旨在确定：1）博士前临床诊所中光激励荧光板（PSP）的微生物污染率及来源；2）每周气体灭菌和屏障方案的组合能否防止PSP板受到污染。
选择50块临床使用的板和25块气体灭菌的对照板进行检查。将PSP板压在血琼脂培养基上，于37℃孵育。记录所得菌落的数量、大小、分布和种类。为了测试这些细菌是否可能来自口腔，选择17个菌落接种在轻唾琼脂（M-S）上进行培养。在M-S琼脂上生长的菌落进行革兰氏染色。
28块测试板（56%）在血琼脂上有细菌菌落生长。其中17个细菌菌落接种在M-S琼脂上，13个（76.47%）生长，其中69%为革兰氏阳性菌。
我们的结果表明，对于口腔内放射摄影，应加强感染控制的标准预防措施，并且每天临床使用后对板进行气体灭菌是一种潜在的解决方案。
