编码人组织型纤溶酶原激活剂的基因的化学合成、克隆及在哺乳动物细胞中的表达。
Chemical synthesis, cloning and expression in mammalian cells of a gene coding for human tissue-type plasminogen activator.
作者信息
Bell L D, Smith J C, Derbyshire R, Finlay M, Johnson I, Gilbert R, Slocombe P, Cook E, Richards H, Clissold P
机构信息
Searle Research and Development, Bucks, U.K.
出版信息
Gene. 1988 Mar 31;63(2):155-63. doi: 10.1016/0378-1119(88)90521-5.
A 1610-bp DNA duplex coding for human tissue-type plasminogen activator has been chemically synthesized using the phosphoramidite procedure, adapted for a custom-built gene synthesizer. The synthesizer, which was designed for both simplicity and speed, permits the rapid construction of relatively large genes and compares favorably in speed with alternative cDNA isolation procedures. The plasminogen activator gene has been expressed in mammalian cells and shown to produce authentic protein by an immuno-activity assay.
利用亚磷酰胺法，在一台定制的基因合成仪上化学合成了一段编码人组织型纤溶酶原激活剂的1610碱基对DNA双链体。该合成仪的设计兼顾了简便性与速度，能够快速构建相对较大的基因，在速度方面与其他cDNA分离方法相比具有优势。纤溶酶原激活剂基因已在哺乳动物细胞中表达，并通过免疫活性测定显示产生了天然蛋白质。
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