Degen S J, Rajput B, Reich E
J Biol Chem. 1986 May 25;261(15):6972-85.
The nucleotide sequence of the human tissue plasminogen activator (t-PA) gene has been established. A total of 36,594 base pairs (bp) was sequenced; this included 32,720 bp from the site of initiation of transcription to the polyadenylation site, in addition to 3,530 and 344 bp of 5' and 3' flanking DNA, respectively. Thirteen intervening sequences divide the gene into 14 coding regions; the size range for exons is 43-914 bp, while that for introns is 111-14,257 bp. The gene and 5' flanking region contain 28 copies of Alu repetitive DNA and a single KpnI repeat. The transcription initiation site was identified by S1 nuclease, exonuclease VII, and primer extension analysis as an A residue; "TATA" and "CAAT" boxes are located in the expected positions upstream of this proposed site. Results of the analysis of the gene sequence and its comparison with data banks are described. The protein and gene structures of tissue and urokinase plasminogen activator are compared; based on these features the evolutionary relationship of the two human plasminogen activators appears to be close.
人类组织型纤溶酶原激活剂（t-PA）基因的核苷酸序列已被确定。共测序了36594个碱基对（bp）；其中包括从转录起始位点到聚腺苷酸化位点的32720 bp，此外还有5'和3'侧翼DNA分别为3530 bp和344 bp。13个间隔序列将该基因分为14个编码区；外显子的大小范围为43 - 914 bp，而内含子的大小范围为111 - 14257 bp。该基因和5'侧翼区域包含28个Alu重复DNA拷贝和一个KpnI重复序列。通过S1核酸酶、核酸外切酶VII和引物延伸分析确定转录起始位点为一个A残基；“TATA”盒和“CAAT”盒位于该推测位点上游的预期位置。描述了基因序列分析结果及其与数据库数据的比较。比较了组织型和尿激酶型纤溶酶原激活剂的蛋白质和基因结构；基于这些特征，两种人类纤溶酶原激活剂的进化关系似乎很近。
