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Scarless assembly of unphosphorylated DNA fragments with a simplified DATEL method.无磷酸化 DNA 片段的无痕组装与简化 DATEL 方法。
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DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase.DATEL：一种使用热稳定核酸外切酶和连接酶的无痕且与序列无关的DNA组装方法。
ACS Synth Biol. 2016 Sep 16;5(9):1028-32. doi: 10.1021/acssynbio.6b00078. Epub 2016 Jun 1.
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DNA Assembly with the DATEL Method.采用DATEL方法进行DNA组装。
Methods Mol Biol. 2018;1772:421-428. doi: 10.1007/978-1-4939-7795-6_24.
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Enzymatic assembly of overlapping DNA fragments.重叠DNA片段的酶促组装
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Characterization and application of aptamers for Taq DNA polymerase selected using an evolution-mimicking algorithm.使用模拟进化算法筛选的针对Taq DNA聚合酶的适配体的表征与应用
Biotechnol Lett. 2006 Dec;28(23):1939-44. doi: 10.1007/s10529-006-9178-4. Epub 2006 Sep 19.
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Sequence-independent cloning methods for long DNA fragments applied to synthetic biology.应用于合成生物学的长DNA片段的序列非依赖克隆方法。
Anal Biochem. 2017 Aug 1;530:5-8. doi: 10.1016/j.ab.2017.04.018. Epub 2017 Apr 28.
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A new thermostable DNA polymerase mixture for efficient amplification of long DNA fragments.一种用于高效扩增长DNA片段的新型热稳定DNA聚合酶混合物。
Prikl Biokhim Mikrobiol. 2010 Mar-Apr;46(2):248-52.
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Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method.双引物非酶促DNA组装：一种高效且准确的多片段DNA组装方法。
Nucleic Acids Res. 2017 Jun 20;45(11):e94. doi: 10.1093/nar/gkx132.
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A longer finger-subdomain of family A DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities.通过宏基因组分析发现的A家族DNA聚合酶更长的指状亚结构域增强了DNA结合和引物延伸能力。
Gene. 2016 Feb 1;576(2 Pt 1):690-5. doi: 10.1016/j.gene.2015.10.030. Epub 2015 Oct 22.
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[Enhancement of hybridization analysis efficacy by means of controlled fragmentation of analyzed DNA].通过对分析的DNA进行可控片段化提高杂交分析效率
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引用本文的文献
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The evolving art of creating genetic diversity: From directed evolution to synthetic biology.创造遗传多样性的演进艺术：从定向进化到合成生物学。
Biotechnol Adv. 2021 Sep-Oct;50:107762. doi: 10.1016/j.biotechadv.2021.107762. Epub 2021 May 15.
本文引用的文献
1
DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase.DATEL：一种使用热稳定核酸外切酶和连接酶的无痕且与序列无关的DNA组装方法。
ACS Synth Biol. 2016 Sep 16;5(9):1028-32. doi: 10.1021/acssynbio.6b00078. Epub 2016 Jun 1.
2
CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA.用于构建兆碱基大小DNA的CasHRA（Cas9辅助的同源重组组装）方法。
Nucleic Acids Res. 2016 Aug 19;44(14):e124. doi: 10.1093/nar/gkw475. Epub 2016 May 24.
3
Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning.通过优化尿嘧啶切除克隆实现精确的DNA组装和基因组工程。
ACS Synth Biol. 2015 Sep 18;4(9):1042-6. doi: 10.1021/acssynbio.5b00113. Epub 2015 Aug 26.
4
Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes.定向进化与合成生物学策略相结合，加速了基因和基因组的半理性工程改造。
Bioengineered. 2015;6(3):136-40. doi: 10.1080/21655979.2015.1011029. Epub 2015 Jan 26.
5
Assembly of highly standardized gene fragments for high-level production of porphyrins in E. coli.用于在大肠杆菌中高水平生产卟啉的高度标准化基因片段的组装。
ACS Synth Biol. 2015 Mar 20;4(3):274-82. doi: 10.1021/sb500055u. Epub 2014 Jun 24.
6
RADOM, an efficient in vivo method for assembling designed DNA fragments up to 10 kb long in Saccharomyces cerevisiae.RADOM，一种在酿酒酵母中高效体内组装长达10 kb设计DNA片段的方法。
ACS Synth Biol. 2015 Mar 20;4(3):213-20. doi: 10.1021/sb500241e. Epub 2014 Jun 4.
7
DNA fragments assembly based on nicking enzyme system.基于切口酶系统的 DNA 片段组装。
PLoS One. 2013;8(3):e57943. doi: 10.1371/journal.pone.0057943. Epub 2013 Mar 6.
8
The MASTER (methylation-assisted tailorable ends rational) ligation method for seamless DNA assembly.MASTER（甲基化辅助可定制末端合理化）连接方法，用于无缝 DNA 组装。
Nucleic Acids Res. 2013 Apr;41(8):e93. doi: 10.1093/nar/gkt122. Epub 2013 Feb 26.
9
GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.GoldenBraid：一种迭代克隆系统，用于标准化组装可重复使用的基因模块。
PLoS One. 2011;6(7):e21622. doi: 10.1371/journal.pone.0021622. Epub 2011 Jul 7.
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A modular cloning system for standardized assembly of multigene constructs.一种用于多基因构建体标准化组装的模块化克隆系统。
PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765.
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