Compagnone-Post P, Turco E, Robinson C, Trucco M
Pittsburgh Cancer Institute, Pennsylvania 15213.
Genomics. 1988 Jan;2(1):8-13. doi: 10.1016/0888-7543(88)90103-6.
The nucleotide sequence of a complete cDNA gene from a DP4-positive HLA-homozygous cell line, PGF, has been determined. This sequence is identical to the exon sequences in a genomic clone derived from another DP4-positive cell line, Priess. In contrast, our DP cDNA sequence shares only limited homology with partial cDNA sequences obtained from clones of three DP4-negative cell lines. On the basis of these results, we conclude that the phenotypic variation of DP alleles is directly attributable to the nucleotide sequence heterogeneity of DP-beta genes. That is, each phenotypic allelic form of DP antigen corresponds to a distinctly different DP-beta gene. Furthermore, this correspondence is found to be unaffected by the markers present at the DQ and DR loci, since the haplotypes of the PGF and Priess cell lines are, respectively, DR2,DQw1,DP4 and DR4,DQw3,DP4.
已确定来自DP4阳性HLA纯合细胞系PGF的一个完整cDNA基因的核苷酸序列。该序列与源自另一个DP4阳性细胞系Priess的基因组克隆中的外显子序列相同。相比之下，我们的DP cDNA序列与从三个DP4阴性细胞系的克隆中获得的部分cDNA序列仅具有有限的同源性。基于这些结果，我们得出结论，DP等位基因的表型变异直接归因于DP-β基因的核苷酸序列异质性。也就是说，DP抗原的每种表型等位基因形式都对应一个明显不同的DP-β基因。此外，发现这种对应关系不受DQ和DR位点上存在的标记的影响，因为PGF和Priess细胞系的单倍型分别是DR2、DQw1、DP4和DR4、DQw3、DP4。
