Woulfe S L, Rice K, Didier D K, Harris M R, Schiffenbauer J, Klearman M, Farrar M A, Schwartz B D
Howard Hughes Medical Institute Laboratories, Washington University School of Medicine, St. Louis, MO.
Hum Immunol. 1989 Sep;26(1):39-45. doi: 10.1016/0198-8859(89)90031-1.
We have previously reported the complete sequence of the cDNAs corresponding to the alleles at the polymorphic loci (DRB1, DRB3, DQA1, DQB1) of the DRw11(5) cell line Swei. We now report the nucleotide and derived amino acid sequence of the alleles at the remaining two polymorphic loci, DPA1 and DPB1, from Swei, which types as DPw4b. Comparison of the DPw4b alpha sequence with previous DP alpha sequences indicates that it is identical to the DP alpha chains of DPw4 and DPw2. However, the DPw4b alpha cDNA encodes a unique 3' untranslated region that corresponds to sequences contained within the last intron of the DP alpha genomic gene. These results indicate that alternative splicing occurs within the Swei DP alpha RNA. Comparison of the DPw4b beta sequence with previously reported DP beta sequences indicates that the first domain is identical to the sequence of the DP beta chain derived from the Burkhardt (Burk) cell line, but that the second domain differs by two amino acids. In addition, a single amino acid difference from DPw2 at position 69 in the first domain of the DPw4b beta chain suggests that this residue is important in determining DP specificity. Furthermore, comparison with other DP beta DNA sequences suggests that variable regions occur at positions 8-11, 55-57, and 84-87.
我们之前报道了与DRw11(5)细胞系Swei的多态性位点（DRB1、DRB3、DQA1、DQB1）等位基因相对应的cDNA的完整序列。我们现在报道来自Swei的其余两个多态性位点DPA1和DPB1等位基因的核苷酸及推导的氨基酸序列，Swei细胞系分型为DPw4b。将DPw4b α序列与之前的DP α序列进行比较表明，它与DPw4和DPw2的DP α链相同。然而，DPw4b α cDNA编码一个独特的3'非翻译区，该区域对应于DP α基因组基因最后一个内含子内包含的序列。这些结果表明，Swei DP α RNA内发生了可变剪接。将DPw4b β序列与之前报道的DP β序列进行比较表明，第一个结构域与源自伯克哈特（Burk）细胞系的DP β链序列相同，但第二个结构域有两个氨基酸不同。此外，DPw4b β链第一个结构域第69位的氨基酸与DPw2有一个氨基酸差异，这表明该残基在决定DP特异性方面很重要。此外，与其他DP β DNA序列的比较表明，可变区出现在第8 - 11位、第55 - 57位和第84 - 87位。
