肌酐定量测定方法。
Methods for Quantitative Creatinine Determination.
作者信息
Moore John F, Sharer J Daniel
机构信息
Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama.
出版信息
Curr Protoc Hum Genet. 2017 Apr 6;93:A.3O.1-A.3O.7. doi: 10.1002/cphg.38.
Reliable measurement of creatinine is necessary to assess kidney function, and also to quantitate drug levels and diagnostic compounds in urine samples. The most commonly used methods are based on the Jaffe principal of alkaline creatinine-picric acid complex color formation. However, other compounds commonly found in serum and urine may interfere with Jaffe creatinine measurements. Therefore, many laboratories have made modifications to the basic method to remove or account for these interfering substances. This appendix will summarize the basic Jaffe method, as well as a modified, automated version. Also described is a high performance liquid chromatography (HPLC) method that separates creatinine from contaminants prior to direct quantification by UV absorption. Lastly, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described that uses stable isotope dilution to reliably quantify creatinine in any sample. This last approach has been recommended by experts in the field as a means to standardize all quantitative creatinine methods against an accepted reference. © 2017 by John Wiley & Sons, Inc.
准确测量肌酐对于评估肾功能、定量尿液样本中的药物水平和诊断化合物都很有必要。最常用的方法基于碱性肌酐-苦味酸复合物显色的Jaffe原理。然而，血清和尿液中常见的其他化合物可能会干扰Jaffe法测定肌酐。因此，许多实验室对基本方法进行了改进，以去除或考虑这些干扰物质。本附录将总结基本的Jaffe法以及一种改进的自动化版本。还介绍了一种高效液相色谱（HPLC）方法，该方法在通过紫外吸收直接定量之前将肌酐与污染物分离。最后，描述了一种液相色谱-串联质谱（LC-MS/MS）方法，该方法使用稳定同位素稀释来可靠地定量任何样本中的肌酐。该领域的专家推荐了这最后一种方法，作为使所有定量肌酐方法与公认参考标准标准化的一种手段。© 2017约翰威立父子公司版权所有
Zotero 插件