喀麦隆常染色体显性多囊肾病患者的肠道微生物群与肾功能：一项横断面研究。

Gut microbiota and kidney function in autosomal dominant polycystic kidney disease participants in Cameroon: a cross-sectional study.

作者信息

Obolo Nwaga Inès, Nzana Victorine Bandolo, Bughe Rhoda Nsen, Dah Isaac, Cho-Fon Cheboh, Mahamat Maimouna, Ndjong Emmanuelle, Nono Aristide, Mballa Jean Claude, Acho Fon Abongwa, Moor Vicky Ama, Mbacham Wilfred Fon, Kaze François Folefack

机构信息

Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.

Yaoundé Central Hospital, Yaoundé, Cameroon.

出版信息

BMC Nephrol. 2025 Jan 13;26(1):20. doi: 10.1186/s12882-025-03942-6.

DOI:10.1186/s12882-025-03942-6

PMID:39806306

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11727510/

Abstract

BACKGROUND AND HYPOTHESIS

Gut dysbiosis characterized by an imbalance in pathobionts (Enterobacter, Escherichia and Salmonella) and symbionts (Bifidobacterium, Lactobacillus and Prevotella) can occur during chronic kidney disease (CKD) progression. We evaluated the associations between representative symbionts (Bifidobacterium and Lactobacillus) and pathobionts (Enterobacteriaceae) with kidney function in persons with autosomal dominant polycystic kidney disease (ADPKD).

METHODS

In this cross-sectional study, 29 ADPKD patients were matched to 15 controls at a 2:1 ratio. Clinical data and biological samples were collected. The estimated glomerular filtration rate (eGFR) was calculated from the serum creatinine concentration using the 2009 Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation. Microbial DNA extracted from stool specimens and amplified by qPCR was used to quantify Enterobacteriaceae, Bifidobacterium and Lactobacillus abundance. Differences between ADPKD subgroups and controls were assessed using nonparametric tests.

RESULTS

The mean age (SD) of the 44 participants was 40.65 (± 11.9) years. Among the participants with ADPKD, 62.1% experienced flank pain, and 48.3% had hypertension. Their median eGFR [IQR] was 74.4 [51.2-94.6] ml/min/1.73m. All stool samples had Enterobacteriaceae. Lactobacillus abundance was lower in ADPKD participants with more pronounced kidney function decline (CKD G3-5: 0.58 ng/μL) than in those with milder damage and controls (G1-2: 0.64 ng/μL, p = 0.047; controls: 0.71 ng/μL, p = 0.043), while Enterobacteriaceae abundance was greater in ADPKD patients with lower kidney function (CKD G3-5: 78.6 ng/μL) than in those in the other two groups (G1-2: 71.6 ng/μL, p = 0.048; controls: 70.5 ng/μL, p = 0.045).

CONCLUSION

Decreased kidney function was associated with decreased symbiont and increased pathobiont abundance in ADPKD patients, suggesting a potential role for the microbiota in disease progression and possible targets for further research.

摘要

背景与假设

以致病共生菌（肠杆菌属、大肠杆菌和沙门氏菌）与共生菌（双歧杆菌属、乳酸杆菌属和普氏菌属）失衡为特征的肠道菌群失调可能在慢性肾脏病（CKD）进展过程中出现。我们评估了常染色体显性多囊肾病（ADPKD）患者中代表性共生菌（双歧杆菌属和乳酸杆菌属）及致病共生菌（肠杆菌科）与肾功能之间的关联。

方法

在这项横断面研究中，29例ADPKD患者与15例对照按2:1的比例进行匹配。收集临床数据和生物样本。使用2009年慢性肾脏病流行病学协作组（CKD-EPI）公式根据血清肌酐浓度计算估计肾小球滤过率（eGFR）。从粪便标本中提取并通过qPCR扩增的微生物DNA用于定量肠杆菌科、双歧杆菌属和乳酸杆菌属的丰度。使用非参数检验评估ADPKD亚组与对照组之间的差异。

结果

44名参与者的平均年龄（标准差）为40.65（±11.9）岁。在ADPKD参与者中，62.1%经历过胁腹痛，48.3%患有高血压。他们的eGFR中位数[四分位间距]为74.4[51.2 - 94.6]ml/min/1.73m²。所有粪便样本中均有肠杆菌科。肾功能下降更明显的ADPKD参与者（CKD G3 - 5：0.58 ng/μL）中乳酸杆菌属丰度低于肾功能损害较轻者及对照组（G1 - 2：0.64 ng/μL，p = 0.047；对照组：0.71 ng/μL，p = 0.043），而肾功能较低的ADPKD患者（CKD G3 - 5：78.6 ng/μL）中肠杆菌科丰度高于其他两组（G1 - 2：71.6 ng/μL，p = 0.048；对照组：70.5 ng/μL，p = 0.045）。