鼠伤寒沙门氏菌中编码磷酸核糖焦磷酸合成酶（prsA）的基因结构。
Structure of the gene encoding phosphoribosylpyrophosphate synthetase (prsA) in Salmonella typhimurium.
作者信息
Bower S G, Hove-Jensen B, Switzer R L
机构信息
Department of Biochemistry, University of Illinois, Urbana 61801.
出版信息
J Bacteriol. 1988 Jul;170(7):3243-8. doi: 10.1128/jb.170.7.3243-3248.1988.
Abstract
The Salmonella typhimurium gene prsA, which encodes phosphoribosylpyrophosphate synthetase, has been cloned, and the nucleotide sequence has been determined. The amino acid sequence derived from the S. typhimurium gene is 99% identical to the derived Escherichia coli sequence and 47% identical to two rat isozyme sequences. Strains containing plasmid-borne prsA have been used to overproduce and purify the enzyme. The promoter for the S. typhimurium prsA gene was identified by deletion analysis and by similarity to the promoter for the E. coli prsA gene. The location of the prsA promoter results in a 416-base-pair 5' untranslated leader in the prsA transcript, which was shown by deletion to be necessary for maximal synthesis of phosphoribosylpyrophosphate synthetase. The S. typhimurium leader contains a 115-base-pair insert relative to the E. coli leader. The insert appears to have no functional significance.
摘要
编码磷酸核糖焦磷酸合成酶的鼠伤寒沙门氏菌基因prsA已被克隆，并测定了其核苷酸序列。从鼠伤寒沙门氏菌基因推导的氨基酸序列与从大肠杆菌推导的序列有99%的同一性，与两个大鼠同工酶序列有47%的同一性。含有质粒携带的prsA的菌株已被用于过量生产和纯化该酶。通过缺失分析以及与大肠杆菌prsA基因启动子的相似性，确定了鼠伤寒沙门氏菌prsA基因的启动子。prsA启动子的位置导致prsA转录本中有一个416个碱基对的5'非翻译前导序列，通过缺失实验表明该序列对于磷酸核糖焦磷酸合成酶的最大合成是必需的。相对于大肠杆菌前导序列，鼠伤寒沙门氏菌前导序列含有一个115个碱基对的插入片段。该插入片段似乎没有功能意义。
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