Rajesh Deepa, Chowdappa Chaitra, Gurumurthy Rajesh, Kutty A V Moideen, Balakrishna Sharath
Research Assistant, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
Postgraduate Student, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
J Clin Diagn Res. 2017 Feb;11(2):GC01-GC04. doi: 10.7860/JCDR/2017/24909.9411. Epub 2017 Feb 1.
Tumour necrosis factor-alpha (TNFα) gene -308G/A polymorphism (rs1800629) are associated with psoriasis in several populations worldwide. Presently, there is no literature on the status of this polymorphism in the South Indian population.
To determine the profile of TNFα -308G/A polymorphism among psoriatic patients.
This case-control study involved 74 patients with Psoriasis Vulgaris (PsV) and 74 age and gender matched healthy individuals. Patients were recruited from the Department of Dermatology of R.L. Jalappa Hospital and Research Center, Tamaka, Kolar, Karnataka, India, from March 2014 to March 2016. TNFα -308G/A polymorphism was genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
The frequency of TNFα -308A allele 7.4% among psoriatic and 8.8% among non-psoriatic individuals. The difference was not statistically significant (p=0.82).
Our results indicate that TNFα gene -308G/A polymorphism is not a significant marker for the risk of developing PsV among South Indian (Karnataka) psoriatic patients.
肿瘤坏死因子-α（TNFα）基因-308G/A多态性（rs1800629）在全球多个群体中与银屑病相关。目前，关于该多态性在南印度人群中的情况尚无文献报道。
确定银屑病患者中TNFα -308G/A多态性的概况。
本病例对照研究纳入了74例寻常型银屑病（PsV）患者以及74例年龄和性别匹配的健康个体。患者于2014年3月至2016年3月从印度卡纳塔克邦科拉尔塔马卡的R.L.贾拉帕医院和研究中心皮肤科招募。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对TNFα -308G/A多态性进行基因分型。
银屑病患者中TNFα -308A等位基因频率为7.4%，非银屑病个体中为8.8%。差异无统计学意义（p = 0.82）。
我们的结果表明，在南印度（卡纳塔克邦）银屑病患者中，TNFα基因-308G/A多态性不是发生PsV风险的显著标志物。
