Archambault D, East N, Perk K, Dahlberg J E
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
J Clin Microbiol. 1988 May;26(5):971-5. doi: 10.1128/jcm.26.5.971-975.1988.
Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relatively low sensitivity or because of incomplete cross-reactivity of goat sera with heterologous test antigens. An enzyme-linked immunosorbent assay (ELISA) with purified CAEV antigen and biotin-avidin amplification steps was therefore developed and compared with a radioimmunoassay (RIA) against CAEV p28. Of over 500 sera tested, there was 99% concordance between the two tests. On the other hand, 23 of 24 sera obtained from animals with clinical signs of disease that were negative by agar gel immunodiffusion test (with ovine progressive pneumonia virus antigen) were positive by ELISA and RIA. These results suggest that an ELISA with CAEV antigen is superior to the agar gel immunodiffusion test and is easier and faster than an RIA, and therefore may be the method of choice for diagnosing CAEV infection.
由于感染山羊关节炎-脑炎病毒（CAEV）的动物中表现出疾病临床症状的相对较少，控制和根除该病毒的努力将在很大程度上依赖于一种易于实施的灵敏诊断测试。目前使用的测试实用性有限，这是因为灵敏度相对较低，或者因为山羊血清与异源测试抗原的交叉反应不完全。因此，开发了一种带有纯化CAEV抗原和生物素-抗生物素蛋白放大步骤的酶联免疫吸附测定（ELISA），并将其与针对CAEV p28的放射免疫测定（RIA）进行比较。在测试的500多份血清中，两种测试之间的一致性为99%。另一方面，从有疾病临床症状的动物身上获得的24份血清中，有23份经琼脂凝胶免疫扩散试验（用绵羊进行性肺炎病毒抗原）检测为阴性，但经ELISA和RIA检测为阳性。这些结果表明，带有CAEV抗原的ELISA优于琼脂凝胶免疫扩散试验，并且比RIA更简便快捷，因此可能是诊断CAEV感染的首选方法。
